Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Broadening Adenoviral Oncolysis in PDAC: Interrogation of Patient-Derived Organoids for personalized virotherapy and modulation of miRNA content to boost adenoviral potency |
| Συγγραφείς: |
Raimondi, Giulia |
| Συνεισφορές: |
University/Department: Universitat de Barcelona. Facultat de Farmàcia i Ciències de l'Alimentació |
| Thesis Advisors: |
Fillat i Fonts, Cristina, Noé Mata, Verónica |
| Πηγή: |
TDX (Tesis Doctorals en Xarxa) |
| Στοιχεία εκδότη: |
Universitat de Barcelona, 2020. |
| Έτος έκδοσης: |
2020 |
| Φυσική περιγραφή: |
271 p. |
| Θεματικοί όροι: |
Oncologia, Oncología, Oncology, Adenovirus, Adenoviruses, Micro RNAs, MicroRNAs, Cultius cel·lulars humans, Cultivos celulares humanos, Human cell culture, Ciències de la Salut |
| Περιγραφή: |
The general goal of this thesis has been to progress oncolytic adenovirus therapy for PDAC, by the incorporation of novel preclinical models to test for patient-specific responses and the generation of oncolytic adenoviruses with enhanced therapeutic index. The two main objectives have been the following: i) Evaluate patients-derived organoids (PDOs) technology as a platform to screen for personalized virotherapy in vitro 1) Establishment of a battery of PDOs from PDAC and normal pancreatic tissues, and evaluation of their applicability in the study of adenoviral infection; 2) Screening of a battery of PDOs to identify individual sensitivities to virotherapies, and the effects derived from the combination with chemotherapy; 3) Study virotherapy-responses in metastasis originated from PDOs xenografted in mice; (ii) Improve oncolytic adenovirus potency by modulation of miRNAs deregulated in PDAC 4) Screening of aberrantly expressed miRNAs sensitizing viral oncolysis in PDAC via CRISPR/Cas9 system; 5) Generation of a miRNA sponge-adenovirus and evaluation of its oncolytic effects in vitro and in vivo; 6) Modulation of miRNA levels with the THZ1 transcriptional inhibitor, and assessment of the effects of its combination with oncolytic adenoviruses. |
| Τύπος εγγράφου: |
Dissertation/Thesis |
| Περιγραφή αρχείου: |
application/pdf |
| Γλώσσα: |
English |
| Σύνδεσμος πρόσβασης: |
http://hdl.handle.net/10803/671205 |
| Rights: |
L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by/4.0/ |
| Αριθμός Καταχώρησης: |
edstdx.10803.671205 |
| Βάση Δεδομένων: |
TDX |