Academic Journal
Reduced expression of bZIP19 and bZIP23 increases zinc and cadmium accumulation in Arabidopsis halleri.
| Τίτλος: | Reduced expression of bZIP19 and bZIP23 increases zinc and cadmium accumulation in Arabidopsis halleri. |
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| Συγγραφείς: | Spielmann, Julien, Schloesser, Marie, Hanikenne, Marc |
| Πηγή: | Plant, Cell and Environment, 47 (6), 2093 - 2108 (2024-06) |
| Στοιχεία εκδότη: | John Wiley and Sons Inc, 2024. |
| Έτος έκδοσης: | 2024 |
| Θεματικοί όροι: | ZIP, hyperaccumulation, metal toxicity, metal transport, transcription factor, Arabidopsis Proteins, Basic-Leucine Zipper Transcription Factors, Cadmium, MicroRNAs, Zinc, bZIP23 protein, Arabidopsis, bZIP19 protein, Arabidopsis, MicroRNAs/metabolism, MicroRNAs/genetics, Plant Roots/metabolism, Plant Roots/genetics, Plant Shoots/metabolism, Plant Shoots/genetics, Arabidopsis/metabolism, Arabidopsis/genetics, Arabidopsis Proteins/metabolism, Arabidopsis Proteins/genetics, Basic-Leucine Zipper Transcription Factors/metabolism, Basic-Leucine Zipper Transcription Factors/genetics, Cadmium/metabolism, Gene Expression Regulation, Plant, Zinc/metabolism, Arabidopsis, Plant Roots, Plant Shoots, Physiology, Plant Science, Life sciences, Phytobiology (plant sciences, forestry, mycology...), Sciences du vivant, Biologie végétale (sciences végétales, sylviculture, mycologie...) |
| Περιγραφή: | Zinc is an essential micronutrient for all living organisms. When challenged by zinc-limiting conditions, Arabidopsis thaliana plants use a strategy centered on two transcription factors, bZIP19 and bZIP23, to enhance the expression of several zinc transporters to improve their zinc uptake capacity. In the zinc and cadmium hyperaccumulator plant Arabidopsis halleri, highly efficient root-to-shoot zinc translocation results in constitutive local zinc deficiency in roots and in constitutive high expression of zinc deficiency-responsive ZIP genes, supposedly boosting zinc uptake and accumulation. Here, to disrupt this process and to analyze the functions of AhbZIP19, AhbZIP23 and their target genes in hyperaccumulation, the genes encoding both transcriptional factors were knocked down using artificial microRNAs (amiRNA). Although AhbZIP19, AhbZIP23, and their ZIP target genes were downregulated, amiRNA lines surprisingly accumulated more zinc and cadmium compared to control lines in both roots and shoot driving to shoot toxicity symptoms. These observations suggested the existence of a substitute metal uptake machinery in A. halleri to maintain hyperaccumulation. We propose that the iron uptake transporter AhIRT1 participates in this alternative pathway in A. halleri. |
| Τύπος εγγράφου: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| Γλώσσα: | English |
| Relation: | https://onlinelibrary.wiley.com/doi/pdf/10.1111/pce.14862; urn:issn:0140-7791; urn:issn:1365-3040 |
| DOI: | 10.1111/pce.14862 |
| Σύνδεσμος πρόσβασης: | https://orbi.uliege.be/handle/2268/319417 |
| Rights: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| Αριθμός Καταχώρησης: | edsorb.319417 |
| Βάση Δεδομένων: | ORBi |
| DOI: | 10.1111/pce.14862 |
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