Mixed Materials
An Overview of Epitopes and Methods for Antibody Epitope Mapping
| Title: | An Overview of Epitopes and Methods for Antibody Epitope Mapping |
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| Authors: | Nilvebrant, Johan, Thalén, Niklas Berndt, Rockberg, Johan, Malm, Magdalena |
| Publisher Information: | KTH, Proteinteknologi KTH, Läkemedelsutveckling Springer Nature 2025 |
| Document Type: | Mixed materials |
| Abstract: | Antibodies are protein molecules indispensable for many therapeutic, diagnostic, and research purposes due to their exquisite ability to selectively recognize and bind a given antigen. The particular area of the antigen recognized by the antibody is called the epitope, and mapping of such epitopes can provide important mechanistic insights and indicate for what applications an antibody might be useful. Even though the nature of epitopes can be complex, epitopes in protein antigens are broadly grouped into linear or discontinuous epitopes depending on the positioning of the epitope residues in the antigen sequence and the requirement of structure. Specialized methods for mapping the two different classes of epitopes have been developed. While different in their detail, all of the experimental methods rely on assessing the binding of the antibody to the antigen or a set of antigen mimics. Classical epitope mapping methods, utilizing truncated proteins, small numbers of synthesized peptides, and structural analyses of antibody-antigen complexes, have been significantly refined. Current state-of-the-art methods involve combinations of mutational scanning, protein display, and high throughput screening in conjunction with bioinformatic analyses of large datasets. In line with the significant development of in silico protein structure prediction in recent years, computerized approaches for epitope prediction have also matured considerably. QC 20260311 |
| Index Terms: | Antibody, Antigen, Epitope, Epitope mapping, Peptide, Prediction, Structural biology, Medical Biotechnology (Focus on Cell Biology, (incl. Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy), Medicinsk bioteknologi (Inriktn. mot cellbiologi (inkl. stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci), Chapter in book, info:eu-repo/semantics/bookPart, text |
| DOI: | 10.1007.978-1-0716-4591-8_1 |
| URL: | Methods in Molecular Biology, p. 1-11 |
| Availability: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| Note: | English |
| Other Numbers: | UPE oai:DiVA.org:kth-377724 0000-0002-6104-6446 0000-0002-9728-2889 0000-0002-9977-5724 0000-0003-1763-9073 doi:10.1007/978-1-0716-4591-8_1 PMID 40593411 ISI:001654594200002 Scopus 2-s2.0-105010289133 1581980944 |
| Contributing Source: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| Accession Number: | edsoai.on1581980944 |
| Database: | OAIster |
| DOI: | 10.1007.978-1-0716-4591-8_1 |
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