Mixed Materials
Combination of Doxorubicin and Antiangiogenic Agents in Drug-Eluting Beads: In Vitro Loading and Release Dynamics in View of a Novel Therapeutic Approach for Hepatocellular Carcinoma
| Τίτλος: | Combination of Doxorubicin and Antiangiogenic Agents in Drug-Eluting Beads: In Vitro Loading and Release Dynamics in View of a Novel Therapeutic Approach for Hepatocellular Carcinoma |
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| Additional Titles: | Purpose: Antiangiogenic agents have been used for many years as a first-line systemic treatment for advanced HCC. Embolization with cytostatic drugs on the other hand is the first-line treatment for intermediate HCC. The two types of drugs have not been combined for intraarterial delivery yet. The loading and release dynamics and the in vitro effect of their combination are tested in this experimental study. Materials and Methods: Drug-eluting beads were loaded with doxorubicin, sunitinib and sunitinib analogue piperazine (SAP) alone and with their combinations. Diameter change, loading, release, and effect in cellular proliferation were assessed. Results: The average microsphere diameter after loading was 473.7 µm (μm) for Doxorubicin, 388.4 μm for Sunitinib, 515.5 μm for SAP, 414.8 μm for the combination Doxorubicin/Sunitinib and 468.8 μm for the combination Doxorubicin /SAP. Drug release in 0.9% NaCl was 10% for Doxorubicin, 49% for Sunitinib, 25% for SAP, 20%/18% for the combination Doxorubicin/Sunitinib, and 18%/23% for the combination Doxorubicin/SAP whereas in human plasma it was 56%, 27%, 13%, 76%/63% and 62%/15%, respectively. The mean concentration of Doxorubicin that led to inhibition of 50% of cellular proliferation in an HCC Huh7 cell line was 163.1 nM (nM), for Sunitinib 10.3 micromolar (μΜ), for SAP 16.7 μΜ, for Doxorubicin/Sunitinib 222.4 nM and for Doxorubicin/SAP 275 nM. Conclusions: Doxorubicin may be combined with antiangiogenic drugs with satisfactory in vitro loading and release outcomes and effect on cellular lines. Graphical Abstract: (Figure presented.) © Springer Science+Business Media, LLC, part of Springer Nature and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE) 2024. |
| Συγγραφείς: | Krokidis, Miltiadis, Fakitsa, Danae, Malagari, Katerina, Karampelas, Theodoros, Fokas, Demosthenes, Tamvakopoulos, Constantin, Chatziioannou, Achilles |
| Στοιχεία εκδότη: | 2024 |
| Τύπος εγγράφου: | Mixed materials |
| Όροι ευρετηρίου: | scientific_publication_article, Scientific publication - Journal Article |
| Σύνδεσμος: | |
| Διαθεσιμότητα: | Open access content. Open access content Creative Commons Attribution-NonCommercial 4.0 (CC-BY-NC) |
| Σημείωση: | English |
| Other Numbers: | GRNKU oai:lib.uoa.gr:uoadl:3492224 uoadl:3492224 1523429306 |
| Πηγή συνεισφοράς: | NATIONAL & KAPODISTRIAN UNIV OF ATHENS From OAIster®, provided by the OCLC Cooperative. |
| Αριθμός Καταχώρησης: | edsoai.on1523429306 |
| Βάση Δεδομένων: | OAIster |
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