Book
A highly conserved gene island of three genes on chromosome 3B of hexaploid wheat: diverse gene function and genomic structure maintained in a tightly linked block
| Τίτλος: | A highly conserved gene island of three genes on chromosome 3B of hexaploid wheat: diverse gene function and genomic structure maintained in a tightly linked block |
|---|---|
| Συγγραφείς: | Breen, J, Wicker, T, Kong, X, Juncheng, Z, Ma, W, Feuillet, C, Paux, E, Appels, R, Bellgard, M |
| Πηγή: | Breen, J; Wicker, T; Kong, X; Juncheng, Z; Ma, W; Feuillet, C; Paux, E; Appels, R; Bellgard, M (2010). A highly conserved gene island of three genes on chromosome 3B of hexaploid wheat: diverse gene function and genomic structure maintained in a tightly linked block. BMC Plant Biology, 10:98. |
| Στοιχεία εκδότη: | BioMed Central 2010 |
| Τύπος εγγράφου: | Monograph |
| Περίληψη: | BACKGROUND: The complexity of the wheat genome has resulted from waves of retrotransposable element insertions. Gene deletions and disruptions generated by the fast replacement of repetitive elements in wheat have resulted in disruption of colinearity at a micro (sub-megabase) level among the cereals. In view of genomic changes that are possible within a given time span, conservation of genes between species tends to imply an important functional or regional constraint that does not permit a change in genomic structure. The ctg1034 contig completed in this paper was initially studied because it was assigned to the Sr2 resistance locus region, but detailed mapping studies subsequently assigned it to the long arm of 3B and revealed its unusual features. RESULTS: BAC shotgun sequencing of the hexaploid wheat (Triticum aestivum cv. Chinese Spring) genome has been used to assemble a group of 15 wheat BACs from the chromosome 3B physical map FPC contig ctg1034 into a 783,553 bp genomic sequence. This ctg1034 sequence was annotated for biological features such as genes and transposable elements. A three-gene island was identified among >80% repetitive DNA sequence. Using bioinformatics analysis there were no observable similarity in their gene functions. The ctg1034 gene island also displayed complete conservation of gene order and orientation with syntenic gene islands found in publicly available genome sequences of Brachypodium distachyon, Oryza sativa, Sorghum bicolor and Zea mays, even though the intergenic space and introns were divergent. CONCLUSION: We propose that ctg1034 is located within the heterochromatic C-band region of deletion bin 3BL7 based on the identification of heterochromatic tandem repeats and presence of significant matches to chromodomain-containing gypsy LTR retrotransposable elements. We also speculate that this location, among other highly repetitive sequences, may account for the relative stability in gene order and orientation within the gene |
| Όροι ευρετηρίου: | Department of Plant and Microbial Biology, 580 Plants (Botany), Amino Acid Sequence, Chromosome Mapping, Chromosomes, Artificial, Bacterial, Chromosomes, Plant, genetics, Conserved Sequence, genetics, DNA Transposable Elements, genetics, Genes, Plant, genetics, Genome, Plant, genetics, Molecular Sequence Data, Phenotype, Phylogeny, Plant Proteins, chemistry/genetics/metabolism, Poaceae, classification/genetics, Polyploidy, Repetitive Sequences, Nucleic Acid, genetics, Sequence Alignment, Synteny, genetics, Triticum, classification/genetics, Journal Article, PeerReviewed, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion |
| Σύνδεσμος: | 10.1186/1471-2229-10-98 |
| Διαθεσιμότητα: | Open access content. Open access content info:eu-repo/semantics/openAccess Creative Commons: Attribution 2.0 Generic (CC BY 2.0) http://creativecommons.org/licenses/by/2.0 info:eu-repo/semantics/openAccess |
| Σημείωση: | application/pdf info:doi/10.5167/uzh-43467 English |
| Other Numbers: | CHUZH oai:www.zora.uzh.ch:43467 https://www.zora.uzh.ch/id/eprint/43467/11/Breen2010_V.pdf info:doi/10.5167/uzh-43467 info:doi/10.1186/1471-2229-10-98 info:pmid/20507561 urn:issn:1471-2229 733374331 |
| Πηγή συνεισφοράς: | HAUPTBIBLIOTHEK UNIV OF ZURICH From OAIster®, provided by the OCLC Cooperative. |
| Αριθμός Καταχώρησης: | edsoai.ocn733374331 |
| Βάση Δεδομένων: | OAIster |
| Η περιγραφή δεν είναι διαθέσιμη |