Academic Journal
Establishment and Application of Mismatch Amplification Mutation Assay-PCR for Rapid Detection and Differentiation of Duck Hepatitis A Virus-1 Attenuated Vaccine and Wild Strains
| Τίτλος: | Establishment and Application of Mismatch Amplification Mutation Assay-PCR for Rapid Detection and Differentiation of Duck Hepatitis A Virus-1 Attenuated Vaccine and Wild Strains |
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| Συγγραφείς: | Cheng-Dong Yu, Yu-Ri Choi, Jong-Yeol Park, Sang-Won Kim, Se-Yeoun Cha, Hyung-Kwan Jang, Min Kang, Bai Wei |
| Πηγή: | Animals, Vol 14, Iss 18, p 2733 (2024) |
| Στοιχεία εκδότη: | MDPI AG, 2024. |
| Έτος έκδοσης: | 2024 |
| Συλλογή: | LCC:Veterinary medicine LCC:Zoology |
| Θεματικοί όροι: | DHAV-1, MAMA-PCR, differentiation, wild-type, attenuated vaccine, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| Περιγραφή: | Duck hepatitis A virus type 1 (DHAV-1) is the main pathogen causing viral hepatitis in ducks, marked by high contagion and acute mortality. Live attenuated DHAV-1 vaccines are widely used to control the disease. This study aims to develop a mismatch amplification mutation assay (MAMA)-PCR for the rapid detection and differentiation of Korean DHAV-1 wild-type strains from vaccine strains. A MAMA primer was designed to target a single nucleotide polymorphism (SNPs) at position 2276 within the VP1 gene, allowing differentiation in a single PCR reaction. The MAMA-PCR accurately identified both strains, with detection limits of 100.5 ELD50/mL and 102.3 ELD50/mL, respectively. The MAMA-PCR demonstrated specificity, showing no cross-reactivity with 12 other viral and bacterial pathogens. The MAMA-PCR was applied to 89 farms, yielding results consistent with nested-PCR and sequence determination, identifying four positive farms for DHAV-1 vaccine strains. In conclusion, this study is the first to employ the MAMA-PCR method to distinguish between DHAV-1 wild-type and vaccine strains. The developed method is rapid, simple, specific, and sensitive, thereby serving as an effective tool for clinical diagnostics in identifying and differentiating between Korean DHAV-1 wild-type and vaccine strains. |
| Τύπος εγγράφου: | article |
| Περιγραφή αρχείου: | electronic resource |
| Γλώσσα: | English |
| ISSN: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/14/18/2733; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani14182733 |
| Σύνδεσμος πρόσβασης: | https://doaj.org/article/addcf7b4be004c3daece766283b0a835 |
| Αριθμός Καταχώρησης: | edsdoj.f7b4be004c3daece766283b0a835 |
| Βάση Δεδομένων: | Directory of Open Access Journals |
| ISSN: | 20762615 |
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| DOI: | 10.3390/ani14182733 |