Academic Journal
Protection of Chickens against H9N2 Avian Influenza Isolates with a Live Vector Vaccine Expressing Influenza Hemagglutinin Gene Derived from Y280 Avian Influenza Virus
| Τίτλος: | Protection of Chickens against H9N2 Avian Influenza Isolates with a Live Vector Vaccine Expressing Influenza Hemagglutinin Gene Derived from Y280 Avian Influenza Virus |
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| Συγγραφείς: | Jun-Feng Zhang, Sang-Won Kim, Ke Shang, Jong-Yeol Park, Yu-Ri Choi, Hyung-Kwan Jang, Bai Wei, Min Kang, Se-Yeoun Cha |
| Πηγή: | Animals, Vol 14, Iss 6, p 872 (2024) |
| Στοιχεία εκδότη: | MDPI AG, 2024. |
| Έτος έκδοσης: | 2024 |
| Συλλογή: | LCC:Veterinary medicine LCC:Zoology |
| Θεματικοί όροι: | turkey herpesvirus, CRISPR/Cas9, NHEJ, H9N2, Y280, HA protein, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| Περιγραφή: | Since the outbreak of the H9N2/Y439 avian influenza virus in 1996, the Korean poultry industry has incurred severe economic losses. A novel possibly zoonotic H9N2 virus from the Y280-like lineage (H9N2/Y280) has been prevalent in Korea since June 2020, posing a threat to the poultry sector. Rapid mutation of influenza viruses urges the development of effective vaccines against newly generated strains. Thus, we engineered a recombinant virus rHVT/Y280 to combat H9N2/Y280. We integrated the hemagglutinin (HA) gene of the H9N2/Y280 strain into the US2 region of the herpesvirus of turkeys (HVT) Fc126 vaccine strain, utilizing CRISPR/Cas9 gene-editing technology. The successful construction of rHVT/Y280 was confirmed by polymerase chain reaction and sequencing, followed by efficacy evaluation. Four-day-old specific pathogen-free chickens received the rHVT/Y280 vaccine and were challenged with the H9N2/Y280 strain A21-MRA-003 at 3 weeks post-vaccination. In 5 days, there were no gross lesions among the vaccinated chickens. The rHVT/Y280 vaccine induced strong humoral immunity and markedly reduced virus shedding, achieving 100% inhibition of virus recovery in the cecal tonsil and significantly lowering tissue viral load. Thus, HVT vector vaccines expressing HA can be used for protecting poultry against H9N2/Y280. The induction of humoral immunity by live vaccines is vital in such cases. In summary, the recombinant virus rHVT/Y280 is a promising vaccine candidate for the protection of chickens against the H9N2/Y280. |
| Τύπος εγγράφου: | article |
| Περιγραφή αρχείου: | electronic resource |
| Γλώσσα: | English |
| ISSN: | 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/14/6/872; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani14060872 |
| Σύνδεσμος πρόσβασης: | https://doaj.org/article/7968afc43a534a00a1c755dd80340932 |
| Αριθμός Καταχώρησης: | edsdoj.7968afc43a534a00a1c755dd80340932 |
| Βάση Δεδομένων: | Directory of Open Access Journals |
| ISSN: | 20762615 |
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| DOI: | 10.3390/ani14060872 |