Academic Journal
Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol
| Τίτλος: | Metabolic engineering of Rhodopseudomonas palustris for the obligate reduction of n-butyrate to n-butanol |
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| Συγγραφείς: | Devin F. R. Doud, Eric C. Holmes, Hanno Richter, Bastian Molitor, Georg Jander, Largus T. Angenent |
| Πηγή: | Biotechnology for Biofuels, Vol 10, Iss 1, Pp 1-11 (2017) |
| Στοιχεία εκδότη: | BMC, 2017. |
| Έτος έκδοσης: | 2017 |
| Συλλογή: | LCC:Fuel LCC:Biotechnology |
| Θεματικοί όροι: | Metabolic engineering, Redox-driven obligate reduction, n-Butanol, Rhodopseudomonas palustris, Fuel, TP315-360, Biotechnology, TP248.13-248.65 |
| Περιγραφή: | Abstract Background Rhodopseudomonas palustris is a versatile microbe that encounters an innate redox imbalance while growing photoheterotrophically with reduced substrates. The resulting excess in reducing equivalents, together with ATP from photosynthesis, could be utilized to drive a wide range of bioconversions. The objective of this study was to genetically modify R. palustris to provide a pathway to reduce n-butyrate into n-butanol for maintaining redox balance. Results Here, we constructed and expressed a plasmid-based pathway for n-butanol production from Clostridium acetobutylicum ATCC 824 in R. palustris. We maintained the environmental conditions in such a way that this pathway functioned as the obligate route to re-oxidize excess reducing equivalents, resulting in an innate selection pressure. The engineered strain of R. palustris grew under otherwise restrictive redox conditions and achieved concentrations of 1.5 mM n-butanol at a production rate of 0.03 g L−1 day−1 and a selectivity (i.e., products compared to the consumed substrate) of close to 40%. Since the theoretical maximum selectivity is 45%, the engineered strain converted close to its maximum selectivity. Conclusions The innate redox imbalance of R. palustris can be used to drive the reduction of n-butyrate into n-butanol after expression of a plasmid-based enzyme from a butanol-producing Clostridium strain. |
| Τύπος εγγράφου: | article |
| Περιγραφή αρχείου: | electronic resource |
| Γλώσσα: | English |
| ISSN: | 1754-6834 |
| Relation: | http://link.springer.com/article/10.1186/s13068-017-0864-3; https://doaj.org/toc/1754-6834 |
| DOI: | 10.1186/s13068-017-0864-3 |
| Σύνδεσμος πρόσβασης: | https://doaj.org/article/0d5ae189477a488fa77d8b9b782c6ee8 |
| Αριθμός Καταχώρησης: | edsdoj.0d5ae189477a488fa77d8b9b782c6ee8 |
| Βάση Δεδομένων: | Directory of Open Access Journals |
| ISSN: | 17546834 |
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| DOI: | 10.1186/s13068-017-0864-3 |