| Περιγραφή: |
This thesis develops multiple novel methods spanning computational genomics and machine learning. CRISPR-Cas13d is a programmable RNA-targeting system that can knockdown specific RNA transcripts. Our method, TIGER (Wessels et al., 2023), is the first tool to model CRISPR-Cas13d efficacy as a function of both the target RNA and the guide RNA (gRNA) sequences; doing so enables biologists using our model to design the most effective gRNA for a target transcript, check a gRNA library for any unintended off-target effects, and engineer mismatches between a gRNA and its target to titrate CRISPR-Cas13d efficacy. We leverage TIGER to study CRISPR-Cas13d binding affinity at junction splice sites (Megan D Schertzer et al., 2023) finding CRISPR-Cas13d can uniquely target 89% of human isoforms with high efficacy. Thereafter, we develop two methods (Stirn & Knowles, 2020; Stirn et al., 2023) for generating well-calibrated heteroscedastic variance estimates, which we integrate into TIGER to study sequence-based heteroscedasticity in CRISPR-Cas13d. The goal of single-cell RNA sequencing (scRNA-seq) integration is to isolate technical variation from the biological signals of interest. Several popular integration methods use a semi-supervised variational autoencoding (VAE) framework (Kingma et al., 2014) with partially observed cell-type labels to learn a low-dimensional representation disentangled from technical effects. To better handle partially observed labels in the amortized variational setting, we develop a new distribution on the simplex (Stirn et al., 2019) that mimics the Dirichlet distribution but has analytic reparameterization gradients and thus low gradient variance. Additionally, we develop a novel method for learning structured latent embeddings for the VAE (Kingma & Welling, 2014) that outperforms existing clustering methods on benchmark datasets and state-of-the-art scRNA-seq integration methods when combined with scVI (Lopez et al., 2018), a popular VAE-based scRNA-seq integration method. Our final ... |