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Microrna164D Suppresses the Hvnac92-Hvhkt1;5 Module to Enhance Salinity Tolerance in Barley

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Τίτλος: Microrna164D Suppresses the Hvnac92-Hvhkt1;5 Module to Enhance Salinity Tolerance in Barley
Συγγραφείς: Kuang, Liuhui, Zhou, Hongxing, Zhang, Tongtong, Gao, Fei, Yan, Tao, Chen, Zhong-Hua, Shen, Qiufang, Zhang, Guoping, Li, Lin, Wu, Dezhi
Πηγή: Publications
Στοιχεία εκδότη: Scholar Commons
Έτος έκδοσης: 2025
Συλλογή: University of South Carolina Libraries: Scholar Commons
Θεματικοί όροι: HKT transporter, Na+ translocation, barley, microRNA, salt tolerance, Hordeum (genetics, metabolism, physiology), MicroRNAs (genetics, metabolism), Salt Tolerance (genetics), Plant Proteins (genetics, Gene Expression Regulation, Plant, Sodium (metabolism), Plant Roots (genetics, Potassium (metabolism), Cation Transport Proteins (genetics, Symporters (genetics, Plant Shoots (metabolism, genetics), Nursing
Περιγραφή: Cereal crops (e.g., rice, wheat, maize, and barley) constituted the major component of global human diet and fundamentally changed human society since the dawn of agriculture around 12,000 y ago. Originated and domesticated in different continents and environments, cereal crops vary significantly in their salt tolerance. The High-Affinity K Transporter1;5s (HKT1;5s) predominately regulate Na accumulation and salt tolerance in salt-sensitive cereal crops by mediating shoot-to-root Na exclusion. However, HvHKT1;5 paradoxically promotes root-to-shoot Na translocation in salt-tolerant barley. Therefore, unravelling the regulatory mechanisms of HvHKT1;5 is critical to understanding the molecular basis of salt tolerance in barley. Here, we demonstrated that a microRNA164d-HvNAC92-HvHKT1;5 module improves salt tolerance via reduced shoot Na accumulation and increased K retention in barley, whereas miR164d suppresses HvNAC92 transcription factor to directly downregulate HvHKT1;5 expression. Under salinity condition, the MIR164d-OE, Hvnac92, and Hvhkt1;5 lines showed significantly reduced root-to-shoot Na translocation and shoot Na content compared with the wild-type. In conclusion, we resolve the species-specific function of HKT1;5s in cereal crops by establishing miRNA-guided Na and K transport regulation as a regulatory framework for engineering salt-tolerant crops.
Τύπος εγγράφου: text
Γλώσσα: English
Relation: https://scholarcommons.sc.edu/acorn_pub/838; https://doi.org/10.1073/pnas.2514555122
DOI: 10.1073/pnas.2514555122;
DOI: 10.1073/pnas.2514555122
Διαθεσιμότητα: https://scholarcommons.sc.edu/acorn_pub/838
https://doi.org/10.1073/pnas.2514555122;
https://doi.org/10.1073/pnas.2514555122
Rights: © 2025 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) .
Αριθμός Καταχώρησης: edsbas.F0903ABF
Βάση Δεδομένων: BASE
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  – Url: https://scholarcommons.sc.edu/acorn_pub/838#
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  Data: Microrna164D Suppresses the Hvnac92-Hvhkt1;5 Module to Enhance Salinity Tolerance in Barley
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  Data: <searchLink fieldCode="AR" term="%22Kuang%2C+Liuhui%22">Kuang, Liuhui</searchLink><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Hongxing%22">Zhou, Hongxing</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Tongtong%22">Zhang, Tongtong</searchLink><br /><searchLink fieldCode="AR" term="%22Gao%2C+Fei%22">Gao, Fei</searchLink><br /><searchLink fieldCode="AR" term="%22Yan%2C+Tao%22">Yan, Tao</searchLink><br /><searchLink fieldCode="AR" term="%22Chen%2C+Zhong-Hua%22">Chen, Zhong-Hua</searchLink><br /><searchLink fieldCode="AR" term="%22Shen%2C+Qiufang%22">Shen, Qiufang</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Guoping%22">Zhang, Guoping</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Lin%22">Li, Lin</searchLink><br /><searchLink fieldCode="AR" term="%22Wu%2C+Dezhi%22">Wu, Dezhi</searchLink>
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  Data: 2025
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  Data: <searchLink fieldCode="DE" term="%22HKT+transporter%22">HKT transporter</searchLink><br /><searchLink fieldCode="DE" term="%22Na%2B+translocation%22">Na+ translocation</searchLink><br /><searchLink fieldCode="DE" term="%22barley%22">barley</searchLink><br /><searchLink fieldCode="DE" term="%22microRNA%22">microRNA</searchLink><br /><searchLink fieldCode="DE" term="%22salt+tolerance%22">salt tolerance</searchLink><br /><searchLink fieldCode="DE" term="%22Hordeum+%28genetics%22">Hordeum (genetics</searchLink><br /><searchLink fieldCode="DE" term="%22metabolism%22">metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22physiology%29%22">physiology)</searchLink><br /><searchLink fieldCode="DE" term="%22MicroRNAs+%28genetics%22">MicroRNAs (genetics</searchLink><br /><searchLink fieldCode="DE" term="%22metabolism%29%22">metabolism)</searchLink><br /><searchLink fieldCode="DE" term="%22Salt+Tolerance+%28genetics%29%22">Salt Tolerance (genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+Proteins+%28genetics%22">Plant Proteins (genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+Expression+Regulation%22">Gene Expression Regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Plant%22">Plant</searchLink><br /><searchLink fieldCode="DE" term="%22Sodium+%28metabolism%29%22">Sodium (metabolism)</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+Roots+%28genetics%22">Plant Roots (genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Potassium+%28metabolism%29%22">Potassium (metabolism)</searchLink><br /><searchLink fieldCode="DE" term="%22Cation+Transport+Proteins+%28genetics%22">Cation Transport Proteins (genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Symporters+%28genetics%22">Symporters (genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+Shoots+%28metabolism%22">Plant Shoots (metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22genetics%29%22">genetics)</searchLink><br /><searchLink fieldCode="DE" term="%22Nursing%22">Nursing</searchLink>
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  Data: Cereal crops (e.g., rice, wheat, maize, and barley) constituted the major component of global human diet and fundamentally changed human society since the dawn of agriculture around 12,000 y ago. Originated and domesticated in different continents and environments, cereal crops vary significantly in their salt tolerance. The High-Affinity K Transporter1;5s (HKT1;5s) predominately regulate Na accumulation and salt tolerance in salt-sensitive cereal crops by mediating shoot-to-root Na exclusion. However, HvHKT1;5 paradoxically promotes root-to-shoot Na translocation in salt-tolerant barley. Therefore, unravelling the regulatory mechanisms of HvHKT1;5 is critical to understanding the molecular basis of salt tolerance in barley. Here, we demonstrated that a microRNA164d-HvNAC92-HvHKT1;5 module improves salt tolerance via reduced shoot Na accumulation and increased K retention in barley, whereas miR164d suppresses HvNAC92 transcription factor to directly downregulate HvHKT1;5 expression. Under salinity condition, the MIR164d-OE, Hvnac92, and Hvhkt1;5 lines showed significantly reduced root-to-shoot Na translocation and shoot Na content compared with the wild-type. In conclusion, we resolve the species-specific function of HKT1;5s in cereal crops by establishing miRNA-guided Na and K transport regulation as a regulatory framework for engineering salt-tolerant crops.
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  Data: © 2025 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) .
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