Academic Journal
Microrna164D Suppresses the Hvnac92-Hvhkt1;5 Module to Enhance Salinity Tolerance in Barley
| Τίτλος: | Microrna164D Suppresses the Hvnac92-Hvhkt1;5 Module to Enhance Salinity Tolerance in Barley |
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| Συγγραφείς: | Kuang, Liuhui, Zhou, Hongxing, Zhang, Tongtong, Gao, Fei, Yan, Tao, Chen, Zhong-Hua, Shen, Qiufang, Zhang, Guoping, Li, Lin, Wu, Dezhi |
| Πηγή: | Publications |
| Στοιχεία εκδότη: | Scholar Commons |
| Έτος έκδοσης: | 2025 |
| Συλλογή: | University of South Carolina Libraries: Scholar Commons |
| Θεματικοί όροι: | HKT transporter, Na+ translocation, barley, microRNA, salt tolerance, Hordeum (genetics, metabolism, physiology), MicroRNAs (genetics, metabolism), Salt Tolerance (genetics), Plant Proteins (genetics, Gene Expression Regulation, Plant, Sodium (metabolism), Plant Roots (genetics, Potassium (metabolism), Cation Transport Proteins (genetics, Symporters (genetics, Plant Shoots (metabolism, genetics), Nursing |
| Περιγραφή: | Cereal crops (e.g., rice, wheat, maize, and barley) constituted the major component of global human diet and fundamentally changed human society since the dawn of agriculture around 12,000 y ago. Originated and domesticated in different continents and environments, cereal crops vary significantly in their salt tolerance. The High-Affinity K Transporter1;5s (HKT1;5s) predominately regulate Na accumulation and salt tolerance in salt-sensitive cereal crops by mediating shoot-to-root Na exclusion. However, HvHKT1;5 paradoxically promotes root-to-shoot Na translocation in salt-tolerant barley. Therefore, unravelling the regulatory mechanisms of HvHKT1;5 is critical to understanding the molecular basis of salt tolerance in barley. Here, we demonstrated that a microRNA164d-HvNAC92-HvHKT1;5 module improves salt tolerance via reduced shoot Na accumulation and increased K retention in barley, whereas miR164d suppresses HvNAC92 transcription factor to directly downregulate HvHKT1;5 expression. Under salinity condition, the MIR164d-OE, Hvnac92, and Hvhkt1;5 lines showed significantly reduced root-to-shoot Na translocation and shoot Na content compared with the wild-type. In conclusion, we resolve the species-specific function of HKT1;5s in cereal crops by establishing miRNA-guided Na and K transport regulation as a regulatory framework for engineering salt-tolerant crops. |
| Τύπος εγγράφου: | text |
| Γλώσσα: | English |
| Relation: | https://scholarcommons.sc.edu/acorn_pub/838; https://doi.org/10.1073/pnas.2514555122 |
| DOI: | 10.1073/pnas.2514555122; |
| DOI: | 10.1073/pnas.2514555122 |
| Διαθεσιμότητα: | https://scholarcommons.sc.edu/acorn_pub/838 https://doi.org/10.1073/pnas.2514555122; https://doi.org/10.1073/pnas.2514555122 |
| Rights: | © 2025 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) . |
| Αριθμός Καταχώρησης: | edsbas.F0903ABF |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1073/pnas.2514555122; |
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