Academic Journal
Adjunctive transferrin to reduce the emergence of antibiotic resistance in Gram-negative bacteria
| Τίτλος: | Adjunctive transferrin to reduce the emergence of antibiotic resistance in Gram-negative bacteria |
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| Συγγραφείς: | Luna, Brian M, Ershova, Ksenia, Yan, Jun, Ulhaq, Amber, Nielsen, Travis B, Hsieh, Sarah, Pantapalangkoor, Paul, Vanscoy, Brian, Ambrose, Paul, Rudin, Sue, Hujer, Kristine, Bonomo, Robert A, Actis, Luis, Skaar, Eric P, Spellberg, Brad |
| Συνεισφορές: | National Institute of Allergy and Infectious Diseases, National Institutes of Health, Cleveland Department of Veterans Affairs, Veterans Affairs Merit Review Program, Geriatric Research Education and Clinical Center |
| Πηγή: | Journal of Antimicrobial Chemotherapy ; volume 74, issue 9, page 2631-2639 ; ISSN 0305-7453 1460-2091 |
| Στοιχεία εκδότη: | Oxford University Press (OUP) |
| Έτος έκδοσης: | 2019 |
| Περιγραφή: | Background New strategies are needed to slow the emergence of antibiotic resistance among bacterial pathogens. In particular, society is experiencing a crisis of antibiotic-resistant infections caused by Gram-negative bacterial pathogens and novel therapeutics are desperately needed to combat such diseases. Acquisition of iron from the host is a nearly universal requirement for microbial pathogens—including Gram-negative bacteria—to cause infection. We have previously reported that apo-transferrin (lacking iron) can inhibit the growth of Staphylococcus aureus in culture and diminish emergence of resistance to rifampicin. Objectives To define the potential of apo-transferrin to inhibit in vitro growth of Klebsiella pneumoniae and Acinetobacter baumannii, key Gram-negative pathogens, and to reduce emergence of resistance to antibiotics. Methods The efficacy of apo-transferrin alone or in combination with meropenem or ciprofloxacin against K. pneumoniae and A. baumannii clinical isolates was tested by MIC assay, time–kill assay and assays for the selection of resistant mutants. Results We confirmed that apo-transferrin had detectable MICs for all strains tested of both pathogens. Apo-transferrin mediated an additive antimicrobial effect for both antibiotics against multiple strains in time–kill assays. Finally, adding apo-transferrin to ciprofloxacin or meropenem reduced the emergence of resistant mutants during 20 day serial passaging of both species. Conclusions These results suggest that apo-transferrin may have promise to suppress the emergence of antibiotic-resistant mutants when treating infections caused by Gram-negative bacteria. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| DOI: | 10.1093/jac/dkz225 |
| DOI: | 10.1093/jac/dkz225/28772506/dkz225.pdf |
| Διαθεσιμότητα: | https://doi.org/10.1093/jac/dkz225 http://academic.oup.com/jac/advance-article-pdf/doi/10.1093/jac/dkz225/28772506/dkz225.pdf http://academic.oup.com/jac/article-pdf/74/9/2631/29205764/dkz225.pdf |
| Rights: | http://creativecommons.org/licenses/by-nc/4.0/ |
| Αριθμός Καταχώρησης: | edsbas.E96D9C4E |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1093/jac/dkz225 |
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