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The evaluation of novel biomarkers and antigens for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer).

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Title: The evaluation of novel biomarkers and antigens for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer).
Authors: Goosen, Wynand Johan
Contributors: Miller, Michele, Parsons, S. D. C., Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences: Molecular Biology and Human Genetics.
Publisher Information: Stellenbosch University
Publication Year: 2016
Collection: Stellenbosch University: SUNScholar Research Repository
Subject Terms: Bovine tuberculosis -- Diagnosis, African buffalo -- Diseases, Biomarkers, Bovigam, CFP-10, ESAT-6, Mycobacterium bovis -- Diagnosis, Interferon-gamma induced protein 10, Antigens, UCTD
Description: Thesis (PhD)--Stellenbosch University, 2016. ; ENGLISH SUMMARY: Mycobacterium bovis (M. bovis) forms part of the Mycobacterium tuberculosis complex (MTC), a group of genetically related bacteria that causes tuberculosis in humans and animals. African buffaloes (Syncerus caffer) are maintenance hosts of M. bovis, the causative organism of bovine tuberculosis (bTB). Since this species acts as a bTB reservoir for a wide range of domestic and wildlife species, the detection of M. bovis-infected animals is essential to control spread of the disease. However, diagnostic assays used for bTB management and control programmes, such as the single intradermal comparative tuberculin test (SICTT) and commercially available interferon-gamma release assays (IGRAs), are still believed to be sub-optimal for the diagnosis of bTB in bovids. A potential approach to improving detection of M. bovis infection could be the use of novel diagnostic antigens or the identification of alternative or ancillary biomarkers to interferon-gamma (IFN-γ). The studies presented in this dissertation aim to identify, develop and evaluate novel approaches for improving the detection of M. bovis infection in African buffaloes. The first objective was to evaluate the performance of two new commercially available IGRAs, the Bovigam® PC-EC assay and the Bovigam® PC-HP assay, for the first time in buffaloes and compare their performance to that of two versions of an adapted human IGRA, the modified QuantiFERON® TB-Gold (mQFT) assay. In addition, the effect of increased blood incubation time on sensitivity of the mQFT assay was assessed along with whether centrifugation was a necessary step prior to harvesting the plasma fraction. Furthermore, the relative sensitivities of Bovigam® assays, a modified Bovigam assay that contains an additional stimulation criteria with Mycobacterium fortuitum tuberculin and the SICTT were compared in identified M. bovis-infected buffaloes. Combinations of these assays were evaluated to identify the optimal test algorithm ...
Document Type: thesis
File Description: xv, 104 pages; application/pdf
Language: English
Relation: https://hdl.handle.net/10019.1/100384
Availability: https://hdl.handle.net/10019.1/100384
Rights: Stellenbosch University
Accession Number: edsbas.E6AAC835
Database: BASE
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  – Url: https://hdl.handle.net/10019.1/100384#
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PubType: Dissertation/ Thesis
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IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: The evaluation of novel biomarkers and antigens for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer).
– Name: Author
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  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Goosen%2C+Wynand+Johan%22">Goosen, Wynand Johan</searchLink>
– Name: Author
  Label: Contributors
  Group: Au
  Data: Miller, Michele<br />Parsons, S. D. C.<br />Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences: Molecular Biology and Human Genetics.
– Name: Publisher
  Label: Publisher Information
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  Data: Stellenbosch University
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2016
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  Data: Stellenbosch University: SUNScholar Research Repository
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  Data: <searchLink fieldCode="DE" term="%22Bovine+tuberculosis+--+Diagnosis%22">Bovine tuberculosis -- Diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22African+buffalo+--+Diseases%22">African buffalo -- Diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Bovigam%22">Bovigam</searchLink><br /><searchLink fieldCode="DE" term="%22CFP-10%22">CFP-10</searchLink><br /><searchLink fieldCode="DE" term="%22ESAT-6%22">ESAT-6</searchLink><br /><searchLink fieldCode="DE" term="%22Mycobacterium+bovis+--+Diagnosis%22">Mycobacterium bovis -- Diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Interferon-gamma+induced+protein+10%22">Interferon-gamma induced protein 10</searchLink><br /><searchLink fieldCode="DE" term="%22Antigens%22">Antigens</searchLink><br /><searchLink fieldCode="DE" term="%22UCTD%22">UCTD</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Thesis (PhD)--Stellenbosch University, 2016. ; ENGLISH SUMMARY: Mycobacterium bovis (M. bovis) forms part of the Mycobacterium tuberculosis complex (MTC), a group of genetically related bacteria that causes tuberculosis in humans and animals. African buffaloes (Syncerus caffer) are maintenance hosts of M. bovis, the causative organism of bovine tuberculosis (bTB). Since this species acts as a bTB reservoir for a wide range of domestic and wildlife species, the detection of M. bovis-infected animals is essential to control spread of the disease. However, diagnostic assays used for bTB management and control programmes, such as the single intradermal comparative tuberculin test (SICTT) and commercially available interferon-gamma release assays (IGRAs), are still believed to be sub-optimal for the diagnosis of bTB in bovids. A potential approach to improving detection of M. bovis infection could be the use of novel diagnostic antigens or the identification of alternative or ancillary biomarkers to interferon-gamma (IFN-γ). The studies presented in this dissertation aim to identify, develop and evaluate novel approaches for improving the detection of M. bovis infection in African buffaloes. The first objective was to evaluate the performance of two new commercially available IGRAs, the Bovigam® PC-EC assay and the Bovigam® PC-HP assay, for the first time in buffaloes and compare their performance to that of two versions of an adapted human IGRA, the modified QuantiFERON® TB-Gold (mQFT) assay. In addition, the effect of increased blood incubation time on sensitivity of the mQFT assay was assessed along with whether centrifugation was a necessary step prior to harvesting the plasma fraction. Furthermore, the relative sensitivities of Bovigam® assays, a modified Bovigam assay that contains an additional stimulation criteria with Mycobacterium fortuitum tuberculin and the SICTT were compared in identified M. bovis-infected buffaloes. Combinations of these assays were evaluated to identify the optimal test algorithm ...
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  Data: https://hdl.handle.net/10019.1/100384
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  Data: https://hdl.handle.net/10019.1/100384
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  Data: edsbas.E6AAC835
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RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: Bovine tuberculosis -- Diagnosis
        Type: general
      – SubjectFull: African buffalo -- Diseases
        Type: general
      – SubjectFull: Biomarkers
        Type: general
      – SubjectFull: Bovigam
        Type: general
      – SubjectFull: CFP-10
        Type: general
      – SubjectFull: ESAT-6
        Type: general
      – SubjectFull: Mycobacterium bovis -- Diagnosis
        Type: general
      – SubjectFull: Interferon-gamma induced protein 10
        Type: general
      – SubjectFull: Antigens
        Type: general
      – SubjectFull: UCTD
        Type: general
    Titles:
      – TitleFull: The evaluation of novel biomarkers and antigens for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer).
        Type: main
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      – PersonEntity:
          Name:
            NameFull: Goosen, Wynand Johan
      – PersonEntity:
          Name:
            NameFull: Miller, Michele
      – PersonEntity:
          Name:
            NameFull: Parsons, S. D. C.
      – PersonEntity:
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            NameFull: Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences: Molecular Biology and Human Genetics.
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              M: 01
              Type: published
              Y: 2016
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