Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
The evaluation of novel biomarkers and antigens for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer). |
| Συγγραφείς: |
Goosen, Wynand Johan |
| Συνεισφορές: |
Miller, Michele, Parsons, S. D. C., Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences: Molecular Biology and Human Genetics. |
| Στοιχεία εκδότη: |
Stellenbosch University |
| Έτος έκδοσης: |
2016 |
| Συλλογή: |
Stellenbosch University: SUNScholar Research Repository |
| Θεματικοί όροι: |
Bovine tuberculosis -- Diagnosis, African buffalo -- Diseases, Biomarkers, Bovigam, CFP-10, ESAT-6, Mycobacterium bovis -- Diagnosis, Interferon-gamma induced protein 10, Antigens, UCTD |
| Περιγραφή: |
Thesis (PhD)--Stellenbosch University, 2016. ; ENGLISH SUMMARY: Mycobacterium bovis (M. bovis) forms part of the Mycobacterium tuberculosis complex (MTC), a group of genetically related bacteria that causes tuberculosis in humans and animals. African buffaloes (Syncerus caffer) are maintenance hosts of M. bovis, the causative organism of bovine tuberculosis (bTB). Since this species acts as a bTB reservoir for a wide range of domestic and wildlife species, the detection of M. bovis-infected animals is essential to control spread of the disease. However, diagnostic assays used for bTB management and control programmes, such as the single intradermal comparative tuberculin test (SICTT) and commercially available interferon-gamma release assays (IGRAs), are still believed to be sub-optimal for the diagnosis of bTB in bovids. A potential approach to improving detection of M. bovis infection could be the use of novel diagnostic antigens or the identification of alternative or ancillary biomarkers to interferon-gamma (IFN-γ). The studies presented in this dissertation aim to identify, develop and evaluate novel approaches for improving the detection of M. bovis infection in African buffaloes. The first objective was to evaluate the performance of two new commercially available IGRAs, the Bovigam® PC-EC assay and the Bovigam® PC-HP assay, for the first time in buffaloes and compare their performance to that of two versions of an adapted human IGRA, the modified QuantiFERON® TB-Gold (mQFT) assay. In addition, the effect of increased blood incubation time on sensitivity of the mQFT assay was assessed along with whether centrifugation was a necessary step prior to harvesting the plasma fraction. Furthermore, the relative sensitivities of Bovigam® assays, a modified Bovigam assay that contains an additional stimulation criteria with Mycobacterium fortuitum tuberculin and the SICTT were compared in identified M. bovis-infected buffaloes. Combinations of these assays were evaluated to identify the optimal test algorithm ... |
| Τύπος εγγράφου: |
thesis |
| Περιγραφή αρχείου: |
xv, 104 pages; application/pdf |
| Γλώσσα: |
English |
| Relation: |
https://hdl.handle.net/10019.1/100384 |
| Διαθεσιμότητα: |
https://hdl.handle.net/10019.1/100384 |
| Rights: |
Stellenbosch University |
| Αριθμός Καταχώρησης: |
edsbas.E6AAC835 |
| Βάση Δεδομένων: |
BASE |