Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
RNA-seq reveals the RNA binding proteins, Hfq and RsmA, play various roles in virulence, antibiotic production and genomic flux in Serratia sp. ATCC 39006 |
| Συγγραφείς: |
Wilf, NM, Reid, AJ, Ramsay, JP, Williamson, NR, Croucher, NJ, Gatto, L, Hester, SS, Goulding, D, Barquist, L, Lilley, KS, Kingsley, RA, Dougan, G, Salmond, GPC |
| Στοιχεία εκδότη: |
BMC |
| Έτος έκδοσης: |
2013 |
| Συλλογή: |
The University of Melbourne: Digital Repository |
| Περιγραφή: |
BACKGROUND: Serratia sp. ATCC 39006 (S39006) is a Gram-negative enterobacterium that is virulent in plant and animal models. It produces a red-pigmented trypyrrole secondary metabolite, prodigiosin (Pig), and a carbapenem antibiotic (Car), as well as the exoenzymes, pectate lyase and cellulase. Secondary metabolite production in this strain is controlled by a complex regulatory network involving quorum sensing (QS). Hfq and RsmA (two RNA binding proteins and major post-transcriptional regulators of gene expression) play opposing roles in the regulation of several key phenotypes within S39006. Prodigiosin and carbapenem production was abolished, and virulence attenuated, in an S39006 ∆hfq mutant, while the converse was observed in an S39006 rsmA transposon insertion mutant. RESULTS: In order to define the complete regulon of Hfq and RsmA, deep sequencing of cDNA libraries (RNA-seq) was used to analyse the whole transcriptome of S39006 ∆hfq and rsmA::Tn mutants. Moreover, we investigated global changes in the proteome using an LC-MS/MS approach. Analysis of differential gene expression showed that Hfq and RsmA directly or indirectly regulate (at the level of RNA) 4% and 19% of the genome, respectively, with some correlation between RNA and protein expression. Pathways affected include those involved in antibiotic regulation, virulence, flagella synthesis, and surfactant production. Although Hfq and RsmA are reported to activate flagellum production in E. coli and an adherent-invasive E. coli hfq mutant was shown to have no flagella by electron microscopy, we found that flagellar production was increased in the S39006 rsmA and hfq mutants. Additionally, deletion of rsmA resulted in greater genomic flux with increased activity of two mobile genetic elements. This was confirmed by qPCR and analysis of rsmA culture supernatant revealed the presence of prophage DNA and phage particles. Finally, expression of a hypothetical protein containing DUF364 increased prodigiosin production and was controlled by a putative 5' ... |
| Τύπος εγγράφου: |
article in journal/newspaper |
| Γλώσσα: |
English |
| ISSN: |
1471-2164 |
| Relation: |
https://hdl.handle.net/11343/260020 |
| Διαθεσιμότητα: |
https://hdl.handle.net/11343/260020 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Αριθμός Καταχώρησης: |
edsbas.D316906B |
| Βάση Δεδομένων: |
BASE |