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Non-targeted analysis of lipidic extracts by high-resolution mass spectrometry to characterise the chemical exposome: Comparison of four clean-up strategies applied to egg

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Τίτλος: Non-targeted analysis of lipidic extracts by high-resolution mass spectrometry to characterise the chemical exposome: Comparison of four clean-up strategies applied to egg
Συγγραφείς: Padioleau, A., Cariou, R., Guiffard, I., Le Bizec, B., Escher, Beate, Antignac, J.-P., Dervilly, G.
Πηγή: ISSN: 1570-0232.
Στοιχεία εκδότη: Elsevier
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 1232;; art. 123963
Έτος έκδοσης: 2023
Θεματικοί όροι: Non-targeted analysis, Clean-up, Lipid removal, High-resolution mass spectrometry, Recoveries, Matrix effects
Περιγραφή: Biota samples are used to monitor chemical stressors and their impact on the ecosystem and to describe dietary chemical exposure. These complex matrices require an extraction step followed by clean-up to avoid damaging sensitive analytical instruments based on chromatography coupled to mass spectrometry. While interest for non-targeted analysis (NTA) is increasing, there is no versatile or generic sample preparation for a wide range of contaminants suitable for a diversity of biotic matrices. Among the contaminants’ variety, persistent contaminants are mostly hydrophobic (mid- to non-polar) and bio-magnify through the lipidic fraction. During their extraction, lipids are generally co-extracted, which may cause matrix effect during the analysis such as hindering the acquired signal. The aim of this study was to evaluate the efficacy of four clean-up methods to selectively remove lipids from extracts prior to NTA. We evaluated (i) gel permeation chromatography (GPC), (ii) Captiva EMR-lipid cartridge (EMR), (iii) sulphuric acid degradation (H2SO4) and (iv) polydimethyl siloxane (PDMS) for their efficiency to remove lipids from hen egg extracts. Gas and liquid chromatography coupled with high-resolution mass spectrometry fitted with either electron ionisation or electrospray ionisation sources operating in positive and negative modes were used to determine the performances of the clean-up methods. A set of 102 chemicals with a wide range of physico-chemical properties that covers the chemical space of mid- to non-polar contaminants, was used to assess and compare recoveries and matrix effects. Matrix effects, that could hinder the mass spectrometer signal, were lower for extracts cleaned-up with H2SO4 than for the ones cleaned-up with PDMS, EMR and GPC. The recoveries were satisfactory for both GPC and EMR while those determined for PDMS and H2SO4 were low due to poor partitioning and degradation/dissociation of the compounds, respectively. The choice of the clean-up methods, among those assessed, should be a ...
Τύπος εγγράφου: article in journal/newspaper
Περιγραφή αρχείου: application/pdf
Γλώσσα: English
Relation: https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28470; https://dx.doi.org/10.1016/j.jchromb.2023.123963
DOI: 10.1016/j.jchromb.2023.123963
Διαθεσιμότητα: https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28470
https://doi.org/10.1016/j.jchromb.2023.123963
Rights: info:eu-repo/semantics/openAccess
Αριθμός Καταχώρησης: edsbas.CF097D96
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  Data: Non-targeted analysis of lipidic extracts by high-resolution mass spectrometry to characterise the chemical exposome: Comparison of four clean-up strategies applied to egg
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  Data: <searchLink fieldCode="AR" term="%22Padioleau%2C+A%2E%22">Padioleau, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Cariou%2C+R%2E%22">Cariou, R.</searchLink><br /><searchLink fieldCode="AR" term="%22Guiffard%2C+I%2E%22">Guiffard, I.</searchLink><br /><searchLink fieldCode="AR" term="%22Le+Bizec%2C+B%2E%22">Le Bizec, B.</searchLink><br /><searchLink fieldCode="AR" term="%22Escher%2C+Beate%22">Escher, Beate</searchLink><br /><searchLink fieldCode="AR" term="%22Antignac%2C+J%2E-P%2E%22">Antignac, J.-P.</searchLink><br /><searchLink fieldCode="AR" term="%22Dervilly%2C+G%2E%22">Dervilly, G.</searchLink>
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  Data: Elsevier<br />Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 1232;; art. 123963
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  Data: 2023
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  Data: <searchLink fieldCode="DE" term="%22Non-targeted+analysis%22">Non-targeted analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Clean-up%22">Clean-up</searchLink><br /><searchLink fieldCode="DE" term="%22Lipid+removal%22">Lipid removal</searchLink><br /><searchLink fieldCode="DE" term="%22High-resolution+mass+spectrometry%22">High-resolution mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Recoveries%22">Recoveries</searchLink><br /><searchLink fieldCode="DE" term="%22Matrix+effects%22">Matrix effects</searchLink>
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  Data: Biota samples are used to monitor chemical stressors and their impact on the ecosystem and to describe dietary chemical exposure. These complex matrices require an extraction step followed by clean-up to avoid damaging sensitive analytical instruments based on chromatography coupled to mass spectrometry. While interest for non-targeted analysis (NTA) is increasing, there is no versatile or generic sample preparation for a wide range of contaminants suitable for a diversity of biotic matrices. Among the contaminants’ variety, persistent contaminants are mostly hydrophobic (mid- to non-polar) and bio-magnify through the lipidic fraction. During their extraction, lipids are generally co-extracted, which may cause matrix effect during the analysis such as hindering the acquired signal. The aim of this study was to evaluate the efficacy of four clean-up methods to selectively remove lipids from extracts prior to NTA. We evaluated (i) gel permeation chromatography (GPC), (ii) Captiva EMR-lipid cartridge (EMR), (iii) sulphuric acid degradation (H2SO4) and (iv) polydimethyl siloxane (PDMS) for their efficiency to remove lipids from hen egg extracts. Gas and liquid chromatography coupled with high-resolution mass spectrometry fitted with either electron ionisation or electrospray ionisation sources operating in positive and negative modes were used to determine the performances of the clean-up methods. A set of 102 chemicals with a wide range of physico-chemical properties that covers the chemical space of mid- to non-polar contaminants, was used to assess and compare recoveries and matrix effects. Matrix effects, that could hinder the mass spectrometer signal, were lower for extracts cleaned-up with H2SO4 than for the ones cleaned-up with PDMS, EMR and GPC. The recoveries were satisfactory for both GPC and EMR while those determined for PDMS and H2SO4 were low due to poor partitioning and degradation/dissociation of the compounds, respectively. The choice of the clean-up methods, among those assessed, should be a ...
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      – Text: English
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      – SubjectFull: Clean-up
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