Academic Journal
Non-targeted analysis of lipidic extracts by high-resolution mass spectrometry to characterise the chemical exposome: Comparison of four clean-up strategies applied to egg
| Title: | Non-targeted analysis of lipidic extracts by high-resolution mass spectrometry to characterise the chemical exposome: Comparison of four clean-up strategies applied to egg |
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| Authors: | Padioleau, A., Cariou, R., Guiffard, I., Le Bizec, B., Escher, Beate, Antignac, J.-P., Dervilly, G. |
| Source: | ISSN: 1570-0232. |
| Publisher Information: | Elsevier Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 1232;; art. 123963 |
| Publication Year: | 2023 |
| Subject Terms: | Non-targeted analysis, Clean-up, Lipid removal, High-resolution mass spectrometry, Recoveries, Matrix effects |
| Description: | Biota samples are used to monitor chemical stressors and their impact on the ecosystem and to describe dietary chemical exposure. These complex matrices require an extraction step followed by clean-up to avoid damaging sensitive analytical instruments based on chromatography coupled to mass spectrometry. While interest for non-targeted analysis (NTA) is increasing, there is no versatile or generic sample preparation for a wide range of contaminants suitable for a diversity of biotic matrices. Among the contaminants’ variety, persistent contaminants are mostly hydrophobic (mid- to non-polar) and bio-magnify through the lipidic fraction. During their extraction, lipids are generally co-extracted, which may cause matrix effect during the analysis such as hindering the acquired signal. The aim of this study was to evaluate the efficacy of four clean-up methods to selectively remove lipids from extracts prior to NTA. We evaluated (i) gel permeation chromatography (GPC), (ii) Captiva EMR-lipid cartridge (EMR), (iii) sulphuric acid degradation (H2SO4) and (iv) polydimethyl siloxane (PDMS) for their efficiency to remove lipids from hen egg extracts. Gas and liquid chromatography coupled with high-resolution mass spectrometry fitted with either electron ionisation or electrospray ionisation sources operating in positive and negative modes were used to determine the performances of the clean-up methods. A set of 102 chemicals with a wide range of physico-chemical properties that covers the chemical space of mid- to non-polar contaminants, was used to assess and compare recoveries and matrix effects. Matrix effects, that could hinder the mass spectrometer signal, were lower for extracts cleaned-up with H2SO4 than for the ones cleaned-up with PDMS, EMR and GPC. The recoveries were satisfactory for both GPC and EMR while those determined for PDMS and H2SO4 were low due to poor partitioning and degradation/dissociation of the compounds, respectively. The choice of the clean-up methods, among those assessed, should be a ... |
| Document Type: | article in journal/newspaper |
| File Description: | application/pdf |
| Language: | English |
| Relation: | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28470; https://dx.doi.org/10.1016/j.jchromb.2023.123963 |
| DOI: | 10.1016/j.jchromb.2023.123963 |
| Availability: | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28470 https://doi.org/10.1016/j.jchromb.2023.123963 |
| Rights: | info:eu-repo/semantics/openAccess |
| Accession Number: | edsbas.CF097D96 |
| Database: | BASE |
| DOI: | 10.1016/j.jchromb.2023.123963 |
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