Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Immunization of BALB/c mice against Helicobacter felis infection with Helicobacter pylori urease |
| Συγγραφείς: |
Michetti, P., Corthesy-Theulaz, I., Davin, C., Haas, R., Vaney, A. C., Heitz, M., Bille, J., Kraehenbuhl, J. P., Saraga, E., Blum, A. L. |
| Έτος έκδοσης: |
1994 |
| Συλλογή: |
Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Θεματικοί όροι: |
Administration, Oral Animals Antibodies, Monoclonal/immunology Cross Reactions Female Gastritis/pathology Helicobacter Infections/*prevention & control Helicobacter pylori/*enzymology *Immunization Mice Mice, Inbred BALB C Recombinant Proteins Stomach/pathology Urease/administration & dosage/classification/*immunology |
| Περιγραφή: |
BACKGROUND/AIMS: Because Helicobacter pylori is a potentially dangerous human pathogen, the protective potential of oral immunization with H. pylori urease and its subunits was evaluated in an animal model. METHODS: Mice were orally immunized with H. pylori sonicate, urease, or recombinant enzymatically inactive urease subunits and then challenged with Helicobacter felis. Control mice were sham-immunized. RESULTS: H. felis colonization was present 5 days after challenge in 9 of 10 sham-immunized, 6 of 9 sonicate-immunized, and 3 of 10 urease-immunized animals (P = 0.031 vs. sham-immunized). Twelve days after challenge, urease B-immunized mice had a weaker colonization than sham-immunized controls, whereas urease A had no effect. After 70 days, most urease A- and urease B-immunized mice had cleared the colonization (10/17: P = 0.0019; 16/20: P = 0.00002 vs. sham-immunized). In urease B-immunized animals, protection was often associated with corpus gastritis. CONCLUSIONS: Oral immunization with H. pylori urease protects mice against H. felis infection. Enzymatically inactive urease A and B subunits contain protective epitopes. It is unclear whether protection depends on the development of a mononuclear inflammatory response in the gastric corpus. Our observations should encourage the development of a human vaccine. |
| Τύπος εγγράφου: |
article in journal/newspaper |
| Γλώσσα: |
unknown |
| ISSN: |
0016-5085 |
| Relation: |
Gastroenterology; https://iris.unil.ch/handle/iris/184358; serval:BIB_B4764088ED61; A1994PJ58600012; 7926454 |
| Διαθεσιμότητα: |
https://iris.unil.ch/handle/iris/184358 |
| Αριθμός Καταχώρησης: |
edsbas.CDDEF8E8 |
| Βάση Δεδομένων: |
BASE |