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Seasonal dynamics, Leishmania diversity, and nanopore-based metabarcoding of blood meal origins in Culicoides spp. in the newly emerging focus of leishmaniasis in Northern Thailand

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Title: Seasonal dynamics, Leishmania diversity, and nanopore-based metabarcoding of blood meal origins in Culicoides spp. in the newly emerging focus of leishmaniasis in Northern Thailand
Authors: Promrangsee, Chulaluk, Sriswasdi, Sira, Sunantaraporn, Sakone, Savigamin, Chatuthanai, Pataradool, Thanapat, Sricharoensuk, Chatchapon, Boonserm, Rungfar, Ampol, Rinnara, Pruenglampoo, Pitchayaporn, Mungthin, Mathirut, Schmidt-Chanasit, Jonas, Siriyasatien, Padet, Preativatanyou, Kanok
Source: http://lobid.org/resources/99370673439106441#!, 17(1):400.
Publication Year: 2024
Collection: Publisso (ZB MED-Publikationsportal Lebenswissenschaften)
Subject Terms: Seasons [MeSH], Killick-Kendrick’s criteria, Leishmaniasis, Visceral/parasitology [MeSH], Leishmaniasis/parasitology [MeSH], Leishmaniasis/epidemiology [MeSH], Leishmania/classification [MeSH], Ceratopogonidae/parasitology [MeSH], Northern Thailand, Host preference, Leishmania/isolation, Genetic Variation [MeSH], Female [MeSH], Humans [MeSH], DNA Barcoding, Taxonomic/methods [MeSH], Visceral/epidemiology [MeSH], Leishmaniasis/transmission [MeSH], Leishmania/genetics [MeSH], Animals [MeSH], Thailand/epidemiology [MeSH], Insect Vectors/classification [MeSH], Nanopores [MeSH], Research, Insect Vectors/parasitology [MeSH], Ceratopogonidae/classification [MeSH], Visceral/transmission [MeSH], Nanopore metabarcoding
Description: Abstract Background Clinical cases of leishmaniasis caused by Leishmania (Mundinia) parasites have been increasingly reported in Southeast Asia, particularly Thailand. Recent evidence has shown that Leishmania (Mundinia) parasites successfully developed into infective metacyclic promastigotes in Culicoides biting midges, strongly supporting their putative role in disease transmission. However, Culicoides diversity, host preference, and Leishmania prevalence in endemic areas remain largely unknown. Methods We investigated the seasonal dynamics, infection prevalence, and blood meal identification of Culicoides collected from the emerging focus of visceral leishmaniasis in Lampang Province, Northern Thailand, during 2021–2023. Midge samples were molecularly screened for Leishmania using SSU rRNA-qPCR and ITS1-PCR, followed by Sanger plasmid sequencing, and parasite haplotype diversity was analyzed. Host blood meal origins were comparatively identified using host-specific Cytb-PCRs and a nanopore-based metabarcoding approach. Results A total of 501 parous and gravid females and 46 blood-engorged ones belonging to at least 17 species of five subgenera (Remmia, Trithecoides, ...
Document Type: article in journal/newspaper
Language: English
Relation: https://repository.publisso.de/resource/frl:6523890; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11414243/
DOI: 10.1186/s13071-024-06487-z
Availability: https://repository.publisso.de/resource/frl:6523890
https://doi.org/10.1186/s13071-024-06487-z
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11414243/
Rights: https://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.C739203B
Database: BASE
Description
DOI:10.1186/s13071-024-06487-z