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Seasonal dynamics, Leishmania diversity, and nanopore-based metabarcoding of blood meal origins in Culicoides spp. in the newly emerging focus of leishmaniasis in Northern Thailand

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Τίτλος: Seasonal dynamics, Leishmania diversity, and nanopore-based metabarcoding of blood meal origins in Culicoides spp. in the newly emerging focus of leishmaniasis in Northern Thailand
Συγγραφείς: Promrangsee, Chulaluk, Sriswasdi, Sira, Sunantaraporn, Sakone, Savigamin, Chatuthanai, Pataradool, Thanapat, Sricharoensuk, Chatchapon, Boonserm, Rungfar, Ampol, Rinnara, Pruenglampoo, Pitchayaporn, Mungthin, Mathirut, Schmidt-Chanasit, Jonas, Siriyasatien, Padet, Preativatanyou, Kanok
Πηγή: http://lobid.org/resources/99370673439106441#!, 17(1):400.
Έτος έκδοσης: 2024
Συλλογή: Publisso (ZB MED-Publikationsportal Lebenswissenschaften)
Θεματικοί όροι: Seasons [MeSH], Killick-Kendrick’s criteria, Leishmaniasis, Visceral/parasitology [MeSH], Leishmaniasis/parasitology [MeSH], Leishmaniasis/epidemiology [MeSH], Leishmania/classification [MeSH], Ceratopogonidae/parasitology [MeSH], Northern Thailand, Host preference, Leishmania/isolation, Genetic Variation [MeSH], Female [MeSH], Humans [MeSH], DNA Barcoding, Taxonomic/methods [MeSH], Visceral/epidemiology [MeSH], Leishmaniasis/transmission [MeSH], Leishmania/genetics [MeSH], Animals [MeSH], Thailand/epidemiology [MeSH], Insect Vectors/classification [MeSH], Nanopores [MeSH], Research, Insect Vectors/parasitology [MeSH], Ceratopogonidae/classification [MeSH], Visceral/transmission [MeSH], Nanopore metabarcoding
Περιγραφή: Abstract Background Clinical cases of leishmaniasis caused by Leishmania (Mundinia) parasites have been increasingly reported in Southeast Asia, particularly Thailand. Recent evidence has shown that Leishmania (Mundinia) parasites successfully developed into infective metacyclic promastigotes in Culicoides biting midges, strongly supporting their putative role in disease transmission. However, Culicoides diversity, host preference, and Leishmania prevalence in endemic areas remain largely unknown. Methods We investigated the seasonal dynamics, infection prevalence, and blood meal identification of Culicoides collected from the emerging focus of visceral leishmaniasis in Lampang Province, Northern Thailand, during 2021–2023. Midge samples were molecularly screened for Leishmania using SSU rRNA-qPCR and ITS1-PCR, followed by Sanger plasmid sequencing, and parasite haplotype diversity was analyzed. Host blood meal origins were comparatively identified using host-specific Cytb-PCRs and a nanopore-based metabarcoding approach. Results A total of 501 parous and gravid females and 46 blood-engorged ones belonging to at least 17 species of five subgenera (Remmia, Trithecoides, ...
Τύπος εγγράφου: article in journal/newspaper
Γλώσσα: English
Relation: https://repository.publisso.de/resource/frl:6523890; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11414243/
DOI: 10.1186/s13071-024-06487-z
Διαθεσιμότητα: https://repository.publisso.de/resource/frl:6523890
https://doi.org/10.1186/s13071-024-06487-z
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11414243/
Rights: https://creativecommons.org/licenses/by/4.0/
Αριθμός Καταχώρησης: edsbas.C739203B
Βάση Δεδομένων: BASE
Περιγραφή
DOI:10.1186/s13071-024-06487-z