Academic Journal
Oral immunization with urease and Escherichia coli heat-labile enterotoxin is safe and immunogenic in Helicobacter pylori-infected adults
| Τίτλος: | Oral immunization with urease and Escherichia coli heat-labile enterotoxin is safe and immunogenic in Helicobacter pylori-infected adults |
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| Συγγραφείς: | Michetti, P., Kreiss, C., Kotloff, K. L., Porta, N., Blanco, J. L., Bachmann, D., Herranz, M., Saldinger, P. F., Corthesy-Theulaz, I., Losonsky, G., Nichols, R., Simon, J., Stolte, M., Ackerman, S., Monath, T. P., Blum, A. L. |
| Έτος έκδοσης: | 1999 |
| Συλλογή: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Θεματικοί όροι: | Administration, Oral Adult Bacterial Toxins/*immunology Bacterial Vaccines/adverse effects/*immunology Double-Blind Method Enterotoxins/*immunology *Escherichia coli Proteins Female Helicobacter Infections/*prevention & control Helicobacter pylori/*immunology Humans Immunization/adverse effects Immunoglobulin A/blood Male Middle Aged Stomach/microbiology Urease/*immunology |
| Περιγραφή: | Background & Aims: Oral immunization with Helicobacter pylori urease can cure Helicobacter infection in animals. As a step toward therapeutic immunization in humans, the safety and immunogenicity of oral immunization with recombinant H. pylori urease were tested in H. pylori-infected adults. Methods: Twenty-six H. pylori-infected volunteers were randomized in a double-blind study to four weekly oral doses of 180, 60, or 20 mg of urease with 5 microg heat-labile enterotoxin of Escherichia coli (LT), LT alone, or placebo. Side effects and immune responses were evaluated weekly after immunization, and gastric biopsy specimens were obtained after 1 month and 6 months for histology and quantitative cultures. Results: Diarrhea was noted in 16 of 24 (66%) of the volunteers who completed the study. Antiurease serum immunoglobulin A titers increased 1. 58-fold +/- 0.37-fold and 3.66-fold +/- 1.5-fold (mean +/- SEM) after immunization with 60 and 180 mg urease, respectively, whereas no change occurred in the placebo +/- LT groups (P = 0.005). Circulating antiurease immunoglobulin A-producing cells increased in volunteers exposed to urease compared with placebo (38.9 +/- 13. 6/10(6) vs. 5.4 +/- 3.1; P = 0.018). Eradication of H. pylori infection was not observed, but urease immunization induced a significant decrease in gastric H. pylori density. Conclusions: H. pylori urease with LT is well tolerated and immunogenic in H. pylori-infected individuals. An improved vaccine formulation may induce curative immunity. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | unknown |
| ISSN: | 0016-5085 |
| Relation: | Gastroenterology; https://iris.unil.ch/handle/iris/47959; serval:BIB_392944F95C4A; 000079372600008 |
| DOI: | 10.1016/S0016-5085(99)70063-6 |
| Διαθεσιμότητα: | https://iris.unil.ch/handle/iris/47959 https://doi.org/10.1016/S0016-5085(99)70063-6 |
| Αριθμός Καταχώρησης: | edsbas.C123EC33 |
| Βάση Δεδομένων: | BASE |
| ISSN: | 00165085 |
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| DOI: | 10.1016/S0016-5085(99)70063-6 |