Dissertation/ Thesis
Προβολή σε EDS

Effects of carbon nanotubes on airway epithelial cells and model lipid bilayers : proteomic and biophysical studies

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Effects of carbon nanotubes on airway epithelial cells and model lipid bilayers : proteomic and biophysical studies
Συγγραφείς: Li, Pin
Συνεισφορές: Blazer-Yost, Bonnie, Witzmann, F. A. (Frank A.), Randall, Stephen Karl, 1953-, Petrache, Horia, Wang, Xianzhong
Έτος έκδοσης: 2014
Συλλογή: Indiana University - Purdue University Indianapolis: IUPUI Scholar Works
Θεματικοί όροι: Carbon Nanotube, Calu-3 cells, DPhPC lipid bilayer, Nanotubes -- Research -- Evaluation -- Analysis, Nanostructured materials -- Research -- Evaluation -- Analysis -- Methodology, Epithelial cells -- Effect of chemicals on, Bilayer lipid membranes -- Biotechnology, Nanostructured materials industry, Organic compounds -- Synthesis, Carbon -- Research -- Toxicology, Airway (Medicine) -- Toxicology, Fullerenes, Protein-protein interactions -- Databases, Proteins -- Analysis -- Data processing, Bioinformatics, Mass spectrometry -- Research -- Methodology, Respiratory mucosa -- Pathophysiology, Proteomics, Biological transport -- Regulation, Oxidative stress -- Physiological effect, Cellular signal transduction, Cell interaction, Biology -- Research -- Data processing, Cells -- Permeability, Ion channels -- Research, Pulmonary toxicology
Περιγραφή: Indiana University-Purdue University Indianapolis (IUPUI) ; Carbon nanomaterials are widely produced and used in industry, medicine and scientific research. To examine the impact of exposure to nanoparticles on human health, the human airway epithelial cell line, Calu-3, was used to evaluate changes in the cellular proteome that could account for alterations in cellular function of airway epithelia after 24 h exposure to 10 μg/mL and 100 ng/mL of two common carbon nanoparticles, singleand multi-wall carbon nanotubes (SWCNT, MWCNT). After exposure to the nanoparticles, label-free quantitative mass spectrometry (LFQMS) was used to study differential protein expression. Ingenuity Pathway Analysis (IPA) was used to conduct a bioinformatics analysis of proteins identified by LFQMS. Interestingly, after exposure to a high concentration (10 μg/mL; 0.4 μg/cm2) of MWCNT or SWCNT, only 8 and 13 proteins, respectively, exhibited changes in abundance. In contrast, the abundance of hundreds of proteins was altered in response to a low concentration (100 ng/mL; 4 ng/cm2) of either CNT. Of the 281 and 282 proteins that were significantly altered in response to MWCNT or SWCNT, respectively, 231 proteins were the same. Bioinformatic analyses found that the proteins common to both kinds of nanotubes are associated with the cellular functions of cell death and survival, cell-to-cell signaling and interaction, cellular assembly and organization, cellular growth and proliferation, infectious disease, molecular transport and protein synthesis. The decrease in expression of the majority proteins suggests a general stress response to protect cells. The STRING database was used to analyze the various functional protein networks. Interestingly, some proteins like cadherin 1 (CDH1), signal transducer and activator of transcription 1 (STAT1), junction plakoglobin (JUP), and apoptosis-associated speck-like protein containing a CARD (PYCARD), appear in several functional categories and tend to be in the center of the networks. This central ...
Τύπος εγγράφου: thesis
Περιγραφή αρχείου: application/pdf
Γλώσσα: English
Relation: https://hdl.handle.net/1805/5968; http://dx.doi.org/10.7912/C2/2180
DOI: 10.7912/C2/2180
Διαθεσιμότητα: https://hdl.handle.net/1805/5968
https://doi.org/10.7912/C2/2180
Αριθμός Καταχώρησης: edsbas.BEC3F104
Βάση Δεδομένων: BASE
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PubType: Dissertation/ Thesis
PubTypeId: dissertation
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Effects of carbon nanotubes on airway epithelial cells and model lipid bilayers : proteomic and biophysical studies
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  Data: <searchLink fieldCode="AR" term="%22Li%2C+Pin%22">Li, Pin</searchLink>
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  Data: Blazer-Yost, Bonnie<br />Witzmann, F. A. (Frank A.)<br />Randall, Stephen Karl, 1953-<br />Petrache, Horia<br />Wang, Xianzhong
– Name: DatePubCY
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  Data: 2014
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  Data: Indiana University - Purdue University Indianapolis: IUPUI Scholar Works
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  Data: <searchLink fieldCode="DE" term="%22Carbon+Nanotube%22">Carbon Nanotube</searchLink><br /><searchLink fieldCode="DE" term="%22Calu-3+cells%22">Calu-3 cells</searchLink><br /><searchLink fieldCode="DE" term="%22DPhPC+lipid+bilayer%22">DPhPC lipid bilayer</searchLink><br /><searchLink fieldCode="DE" term="%22Nanotubes+--+Research+--+Evaluation+--+Analysis%22">Nanotubes -- Research -- Evaluation -- Analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Nanostructured+materials+--+Research+--+Evaluation+--+Analysis+--+Methodology%22">Nanostructured materials -- Research -- Evaluation -- Analysis -- Methodology</searchLink><br /><searchLink fieldCode="DE" term="%22Epithelial+cells+--+Effect+of+chemicals+on%22">Epithelial cells -- Effect of chemicals on</searchLink><br /><searchLink fieldCode="DE" term="%22Bilayer+lipid+membranes+--+Biotechnology%22">Bilayer lipid membranes -- Biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Nanostructured+materials+industry%22">Nanostructured materials industry</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+compounds+--+Synthesis%22">Organic compounds -- Synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+--+Research+--+Toxicology%22">Carbon -- Research -- Toxicology</searchLink><br /><searchLink fieldCode="DE" term="%22Airway+%28Medicine%29+--+Toxicology%22">Airway (Medicine) -- Toxicology</searchLink><br /><searchLink fieldCode="DE" term="%22Fullerenes%22">Fullerenes</searchLink><br /><searchLink fieldCode="DE" term="%22Protein-protein+interactions+--+Databases%22">Protein-protein interactions -- Databases</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins+--+Analysis+--+Data+processing%22">Proteins -- Analysis -- Data processing</searchLink><br /><searchLink fieldCode="DE" term="%22Bioinformatics%22">Bioinformatics</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry+--+Research+--+Methodology%22">Mass spectrometry -- Research -- Methodology</searchLink><br /><searchLink fieldCode="DE" term="%22Respiratory+mucosa+--+Pathophysiology%22">Respiratory mucosa -- Pathophysiology</searchLink><br /><searchLink fieldCode="DE" term="%22Proteomics%22">Proteomics</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+transport+--+Regulation%22">Biological transport -- Regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidative+stress+--+Physiological+effect%22">Oxidative stress -- Physiological effect</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+interaction%22">Cell interaction</searchLink><br /><searchLink fieldCode="DE" term="%22Biology+--+Research+--+Data+processing%22">Biology -- Research -- Data processing</searchLink><br /><searchLink fieldCode="DE" term="%22Cells+--+Permeability%22">Cells -- Permeability</searchLink><br /><searchLink fieldCode="DE" term="%22Ion+channels+--+Research%22">Ion channels -- Research</searchLink><br /><searchLink fieldCode="DE" term="%22Pulmonary+toxicology%22">Pulmonary toxicology</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Indiana University-Purdue University Indianapolis (IUPUI) ; Carbon nanomaterials are widely produced and used in industry, medicine and scientific research. To examine the impact of exposure to nanoparticles on human health, the human airway epithelial cell line, Calu-3, was used to evaluate changes in the cellular proteome that could account for alterations in cellular function of airway epithelia after 24 h exposure to 10 μg/mL and 100 ng/mL of two common carbon nanoparticles, singleand multi-wall carbon nanotubes (SWCNT, MWCNT). After exposure to the nanoparticles, label-free quantitative mass spectrometry (LFQMS) was used to study differential protein expression. Ingenuity Pathway Analysis (IPA) was used to conduct a bioinformatics analysis of proteins identified by LFQMS. Interestingly, after exposure to a high concentration (10 μg/mL; 0.4 μg/cm2) of MWCNT or SWCNT, only 8 and 13 proteins, respectively, exhibited changes in abundance. In contrast, the abundance of hundreds of proteins was altered in response to a low concentration (100 ng/mL; 4 ng/cm2) of either CNT. Of the 281 and 282 proteins that were significantly altered in response to MWCNT or SWCNT, respectively, 231 proteins were the same. Bioinformatic analyses found that the proteins common to both kinds of nanotubes are associated with the cellular functions of cell death and survival, cell-to-cell signaling and interaction, cellular assembly and organization, cellular growth and proliferation, infectious disease, molecular transport and protein synthesis. The decrease in expression of the majority proteins suggests a general stress response to protect cells. The STRING database was used to analyze the various functional protein networks. Interestingly, some proteins like cadherin 1 (CDH1), signal transducer and activator of transcription 1 (STAT1), junction plakoglobin (JUP), and apoptosis-associated speck-like protein containing a CARD (PYCARD), appear in several functional categories and tend to be in the center of the networks. This central ...
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  Data: https://hdl.handle.net/1805/5968; http://dx.doi.org/10.7912/C2/2180
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  Data: 10.7912/C2/2180
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.7912/C2/2180
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: Carbon Nanotube
        Type: general
      – SubjectFull: Calu-3 cells
        Type: general
      – SubjectFull: DPhPC lipid bilayer
        Type: general
      – SubjectFull: Nanotubes -- Research -- Evaluation -- Analysis
        Type: general
      – SubjectFull: Nanostructured materials -- Research -- Evaluation -- Analysis -- Methodology
        Type: general
      – SubjectFull: Epithelial cells -- Effect of chemicals on
        Type: general
      – SubjectFull: Bilayer lipid membranes -- Biotechnology
        Type: general
      – SubjectFull: Nanostructured materials industry
        Type: general
      – SubjectFull: Organic compounds -- Synthesis
        Type: general
      – SubjectFull: Carbon -- Research -- Toxicology
        Type: general
      – SubjectFull: Airway (Medicine) -- Toxicology
        Type: general
      – SubjectFull: Fullerenes
        Type: general
      – SubjectFull: Protein-protein interactions -- Databases
        Type: general
      – SubjectFull: Proteins -- Analysis -- Data processing
        Type: general
      – SubjectFull: Bioinformatics
        Type: general
      – SubjectFull: Mass spectrometry -- Research -- Methodology
        Type: general
      – SubjectFull: Respiratory mucosa -- Pathophysiology
        Type: general
      – SubjectFull: Proteomics
        Type: general
      – SubjectFull: Biological transport -- Regulation
        Type: general
      – SubjectFull: Oxidative stress -- Physiological effect
        Type: general
      – SubjectFull: Cellular signal transduction
        Type: general
      – SubjectFull: Cell interaction
        Type: general
      – SubjectFull: Biology -- Research -- Data processing
        Type: general
      – SubjectFull: Cells -- Permeability
        Type: general
      – SubjectFull: Ion channels -- Research
        Type: general
      – SubjectFull: Pulmonary toxicology
        Type: general
    Titles:
      – TitleFull: Effects of carbon nanotubes on airway epithelial cells and model lipid bilayers : proteomic and biophysical studies
        Type: main
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            NameFull: Li, Pin
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            NameFull: Blazer-Yost, Bonnie
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            NameFull: Wang, Xianzhong
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              M: 01
              Type: published
              Y: 2014
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