Dissertation/ Thesis
Effects of carbon nanotubes on airway epithelial cells and model lipid bilayers : proteomic and biophysical studies
| Τίτλος: | Effects of carbon nanotubes on airway epithelial cells and model lipid bilayers : proteomic and biophysical studies |
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| Συγγραφείς: | Li, Pin |
| Συνεισφορές: | Blazer-Yost, Bonnie, Witzmann, F. A. (Frank A.), Randall, Stephen Karl, 1953-, Petrache, Horia, Wang, Xianzhong |
| Έτος έκδοσης: | 2014 |
| Συλλογή: | Indiana University - Purdue University Indianapolis: IUPUI Scholar Works |
| Θεματικοί όροι: | Carbon Nanotube, Calu-3 cells, DPhPC lipid bilayer, Nanotubes -- Research -- Evaluation -- Analysis, Nanostructured materials -- Research -- Evaluation -- Analysis -- Methodology, Epithelial cells -- Effect of chemicals on, Bilayer lipid membranes -- Biotechnology, Nanostructured materials industry, Organic compounds -- Synthesis, Carbon -- Research -- Toxicology, Airway (Medicine) -- Toxicology, Fullerenes, Protein-protein interactions -- Databases, Proteins -- Analysis -- Data processing, Bioinformatics, Mass spectrometry -- Research -- Methodology, Respiratory mucosa -- Pathophysiology, Proteomics, Biological transport -- Regulation, Oxidative stress -- Physiological effect, Cellular signal transduction, Cell interaction, Biology -- Research -- Data processing, Cells -- Permeability, Ion channels -- Research, Pulmonary toxicology |
| Περιγραφή: | Indiana University-Purdue University Indianapolis (IUPUI) ; Carbon nanomaterials are widely produced and used in industry, medicine and scientific research. To examine the impact of exposure to nanoparticles on human health, the human airway epithelial cell line, Calu-3, was used to evaluate changes in the cellular proteome that could account for alterations in cellular function of airway epithelia after 24 h exposure to 10 μg/mL and 100 ng/mL of two common carbon nanoparticles, singleand multi-wall carbon nanotubes (SWCNT, MWCNT). After exposure to the nanoparticles, label-free quantitative mass spectrometry (LFQMS) was used to study differential protein expression. Ingenuity Pathway Analysis (IPA) was used to conduct a bioinformatics analysis of proteins identified by LFQMS. Interestingly, after exposure to a high concentration (10 μg/mL; 0.4 μg/cm2) of MWCNT or SWCNT, only 8 and 13 proteins, respectively, exhibited changes in abundance. In contrast, the abundance of hundreds of proteins was altered in response to a low concentration (100 ng/mL; 4 ng/cm2) of either CNT. Of the 281 and 282 proteins that were significantly altered in response to MWCNT or SWCNT, respectively, 231 proteins were the same. Bioinformatic analyses found that the proteins common to both kinds of nanotubes are associated with the cellular functions of cell death and survival, cell-to-cell signaling and interaction, cellular assembly and organization, cellular growth and proliferation, infectious disease, molecular transport and protein synthesis. The decrease in expression of the majority proteins suggests a general stress response to protect cells. The STRING database was used to analyze the various functional protein networks. Interestingly, some proteins like cadherin 1 (CDH1), signal transducer and activator of transcription 1 (STAT1), junction plakoglobin (JUP), and apoptosis-associated speck-like protein containing a CARD (PYCARD), appear in several functional categories and tend to be in the center of the networks. This central ... |
| Τύπος εγγράφου: | thesis |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| Relation: | https://hdl.handle.net/1805/5968; http://dx.doi.org/10.7912/C2/2180 |
| DOI: | 10.7912/C2/2180 |
| Διαθεσιμότητα: | https://hdl.handle.net/1805/5968 https://doi.org/10.7912/C2/2180 |
| Αριθμός Καταχώρησης: | edsbas.BEC3F104 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.7912/C2/2180 |
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