Academic Journal
Differential regulation of amidase- and formamidase-mediated ammonia production by the Helicobacter pylori fur repressor.
| Τίτλος: | Differential regulation of amidase- and formamidase-mediated ammonia production by the Helicobacter pylori fur repressor. |
|---|---|
| Συγγραφείς: | Vliet, A.H.M. (Arnoud) van, Stoof, J. (Jeroen), Poppelaars, S.W. (Sophie), Bereswill, S. (Stefan), Homuth, G. (Georg), Kist, M. (Manfred), Kuipers, E.J. (Ernst), Kusters, J.G. (Johannes) |
| Πηγή: | Journal of Biological Chemistry vol. 278 no. 11, pp. 9052-9057 |
| Έτος έκδοσης: | 2003 |
| Συλλογή: | RePub - Publications from Erasmus University, Rotterdam |
| Θεματικοί όροι: | Gene Expression Regulation, Amidohydrolases/*biosynthesis/metabolism, Ammonia/*metabolism, Bacterial Proteins/chemistry/*metabolism, Base Sequence, DNA/metabolism, Helicobacter pylori/*metabolism/pathogenicity, Humans, Iron-Regulatory Proteins/*metabolism, Iron/pharmacology, Models, Biological, Molecular Sequence Data, Nucleic Acid Hybridization, Plasmids/metabolism, Promoter Regions (Genetics), Protein Binding, RNA/metabolism, Repressor Proteins/chemistry/*metabolism, Research Support, Non-U.S. Gov't, Substrate Specificity, Transcription, Genetic, Urease/chemistry |
| Περιγραφή: | The production of high levels of ammonia allows the human gastric pathogen Helicobacter pylori to survive the acidic conditions in the human stomach. H. pylori produces ammonia through urease-mediated degradation of urea, but it is also able to convert a range of amide substrates into ammonia via its AmiE amidase and AmiF formamidase enzymes. Here data are provided that demonstrate that the iron-responsive regulatory protein Fur directly and indirectly regulates the activity of the two H. pylori amidases. In contrast to other amidase-positive bacteria, amidase and formamidase enzyme activities were not induced by medium supplementation with their respective substrates, acrylamide and formamide. AmiE protein expression and amidase enzyme activity were iron-repressed in H. pylori 26695 but constitutive in the isogenic fur mutant. This regulation was mediated at the transcriptional level via the binding of Fur to the amiE promoter region. In contrast, formamidase enzyme activity was not iron-repressed but was significantly higher in the fur mutant. This effect was not mediated at the transcriptional level, and Fur did not bind to the amiF promoter region. These roles of Fur in regulation of the H. pylori amidases suggest that the H. pylori Fur regulator may have acquired extra functions to compensate for the absence of other regulatory systems. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| Relation: | https://repub.eur.nl/pub/13127; urn:hdl:1765/13127 |
| DOI: | 10.1074/jbc.M207542200 |
| Διαθεσιμότητα: | https://repub.eur.nl/pub/13127 https://doi.org/10.1074/jbc.M207542200 |
| Αριθμός Καταχώρησης: | edsbas.B3A856D3 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1074/jbc.M207542200 |
|---|