Academic Journal
Different requirements for alpha- galactosylceramide and recombinant IL-12 antitumor activity in the treatment of C-26 colon carcinoma hepatic metastases.
| Title: | Different requirements for alpha- galactosylceramide and recombinant IL-12 antitumor activity in the treatment of C-26 colon carcinoma hepatic metastases. |
|---|---|
| Authors: | CHIODONI C., SANGALLETTI S., GRI G., CAPETTI B., KOEZUKA Y., COLOMBO M.P., STOPPACCIARO, ANTONELLA |
| Contributors: | Chiodoni, C., Stoppacciaro, Antonella, Sangalletti, S., Gri, G., Capetti, B., Koezuka, Y., Colombo, M. P. |
| Publisher Information: | Wiley-VCH Verlag GmBH:PO Box 101161, D 69451 Weinheim Germany:011 49 6201 606147, EMAIL: service@wiley-vch.de, INTERNET: http://www.wiley-vch.de, Fax: 011 49 6201 606328 |
| Publication Year: | 2001 |
| Collection: | Sapienza Università di Roma: CINECA IRIS |
| Description: | The glycolipid alpha-galactosylceramide (alpha-GalCer), ligand of NKT cells, has been recently shown to induce antitumor immunity in mice through the induction of IL-12 production by dendritic cells. In the present study we compared alpha-GalCer and rIL-12 antitumor activities in the treatment of hepatic metastases of the C-26 murine colon carcinoma. We show that in immunocompetent mice the two molecules display similar efficacy, whereas in mice knockout (KO) for beta2-microglobulin (beta2m), IFN-gamma or IL-12p40, alpha-GalCer antitumor activity is severely impaired. Conversely,in all such KO mice, rIL-12 retains its efficacy. In this context, the IL-12 effect relies on NK cell function since it is abrogated by antibodies to NK1.1, expressed by both NK and NKT cells, but not in beta2m KO mice that lack NKT and CD8 T cells, but have a perfectly functional NK cell population. Furthermore, in IFN-gamma and IL-12p40 double KO mice, exogenous rIL-12 completely loses antitumor efficacy, suggesting the existence of an IFN-gamma-independent IL-12 effect that does require the presence of endogenous IL-12p40 chain. |
| Document Type: | article in journal/newspaper |
| Language: | unknown |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/11592087; info:eu-repo/semantics/altIdentifier/wos/WOS:000171785400030; volume:31; firstpage:3101; lastpage:3110; numberofpages:10; journal:EUROPEAN JOURNAL OF IMMUNOLOGY; https://hdl.handle.net/11573/67451; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000171785400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a |
| Availability: | https://hdl.handle.net/11573/67451 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000171785400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=0c7ff228ccbaaa74236f48834a34396a http://www.scopus.com/inward/record.url?eid=2-s2.0-0034777510&partnerID=65&md5=66802e40a67119de7374c2a6d65a69cf |
| Accession Number: | edsbas.A5447B46 |
| Database: | BASE |
| Description not available. |