Academic Journal
Clostridium perfringens urease genes are plasmid borne.
| Τίτλος: | Clostridium perfringens urease genes are plasmid borne. |
|---|---|
| Συγγραφείς: | Dupuy, B., Daube, G, Popoff, M., Cole, S |
| Συνεισφορές: | Génétique Moléculaire Bactérienne, Institut Pasteur Paris (IP), Faculté de Médecine Vétérinaire Liège, Toxines bactériennes - Bacterial Toxins, This investigation received financial support from the Legs van Straelen and the Institut Pasteur., We are very grateful to Richard Ferrero, Per Einar Granum, Joanna Gilbert, Isabelle Guillouard, Seiichi Katayama, Agne`s Labigne, Harry Mobley, and Linc Sonenshein for kind gifts of strains, reagents, and probes and for helpful discussions. Special thanks to Thierry Garnier for help with bioinformatics. |
| Πηγή: | ISSN: 0019-9567. |
| Στοιχεία εκδότη: | CCSD American Society for Microbiology |
| Έτος έκδοσης: | 1997 |
| Συλλογή: | Institut Pasteur: HAL |
| Θεματικοί όροι: | MESH: Amino Acid Sequence, MESH: Cloning, Molecular, MESH: Urease/analysis, MESH: Urease/genetics, MESH: Clostridium perfringens/enzymology, MESH: Clostridium perfringens/genetics, MESH: Clostridium perfringens/pathogenicity, MESH: Enterotoxins/blood, MESH: Molecular Sequence Data, MESH: Operon, MESH: Plasmids, [SDV]Life Sciences [q-bio], [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology |
| Περιγραφή: | International audience ; Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease phenotype has not been analyzed in the anaerobic pathogen Clostridium perfringens. In this study, approximately 2% of C. perfringens strains, representing the principal biotypes, were found to harbor the urease structural genes, ureABC, and these were localized on large plasmids that often encode, in addition, the lethal epsilon or iota toxins or the enterotoxin. This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. The C. perfringens enzyme was highly similar to the ureases of other bacteria and cross-reacted with antibodies raised against the urease purified from Helicobacter pylori. Urease production was inhibited by urea and induced under growth conditions where the availability of nitrogen sources was limiting. To date, this form of regulation has been observed only for chromosomal ureABC genes. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/9169769; PUBMED: 9169769; PUBMEDCENTRAL: PMC175321 |
| Διαθεσιμότητα: | https://pasteur.hal.science/pasteur-02445974 |
| Αριθμός Καταχώρησης: | edsbas.99DD4820 |
| Βάση Δεδομένων: | BASE |
| Η περιγραφή δεν είναι διαθέσιμη |