Academic Journal
Population genetics analysis of Phlebotomus papatasi sand flies from Egypt and Jordan based on mitochondrial cytochrome b haplotypes
| Τίτλος: | Population genetics analysis of Phlebotomus papatasi sand flies from Egypt and Jordan based on mitochondrial cytochrome b haplotypes |
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| Συγγραφείς: | Flanley, Catherine, Ramalho-Ortigao, Marcelo, Coutinho-Abreu, Iliano, Mukbel, Rami, Hanafi, Hanafi, El-Hossary, Shabaan, Fawaz, Emad El-Din Y., Hoel, David, Bray, Alexander, Stayback, Gwen, Shoue, Douglas, Kamhawi, Shaden, Karakuş, Mehmet, Jaouadi, Kaouther, Yaghoobie-Ershadi, Mohammad Reza, Krüger, Andreas, Amro, Ahmad, Kenawy, Mohamed Amin, Dokhan, Mostafa Ramadhan, Warburg, Alon, Hamarsheh, Omar, Mcdowell, Mary Ann |
| Συνεισφορές: | Eck Institute for Global Health, University of Notre Dame Indiana (UND), Department of Preventive Medicine and Biostatistics Bethesda, Uniformed Services University of the Health Sciences (USUHS), Laboratory of Malaria and Vector Research Rockville, National Institute of Allergy and Infectious Diseases Bethesda (NIAID-NIH), National Institutes of Health Bethesda, MD, USA (NIH)-National Institutes of Health Bethesda, MD, USA (NIH), Faculty of Veterinary Medicine Irbid, Jordan, Jordan University of Science and Technology Irbid, Jordan (JUST), Vector Biology Research Program Cairo, U.S. Naval Medical Research Unit, Ege university Izmir, Turkey, Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection (LR11IPT02), Institut Pasteur de Tunis, Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur), Tehran University of Medical Sciences (TUMS), Department of Tropical Medicine, Military Hospital Hamburg, Al-Quds University East Jerusalem, Palestine, Department of Entomology Cairo, Université Ain Shams, University of Sabratha, Kuvin Center for the Study of Infectious and Tropical Diseases, Hadassah Hebrew University Medical Center Jerusalem, This project was supported by contract No. W911NF0410380 from the Department of Defense (DoD) Defense Advanced Research Projects Agency (DARPA) awarded to MAM. OH was supported by a grant from the Eck Institute for Global Health at the University of Notre Dame, a Fulbright Senior Scientist grant, and Zamallah Academic Development Program. CMF was supported by an Arthur J. Schmitt Leadership Fellowship in Science and Engineering and William and Linda Stavropoulos Fellowship in Science. |
| Πηγή: | ISSN: 1756-3305. |
| Στοιχεία εκδότη: | CCSD BioMed Central |
| Έτος έκδοσης: | 2018 |
| Συλλογή: | Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| Θεματικοί όροι: | mtDNA, Sand flies, Population genetics, Phlebotomus papatasi, Jordan, Haplotypes, Genetic differentiation, Egypt, Cytochrome b, MESH: Animals, MESH: Cytochromes b/genetics, MESH: Insect Vectors/classification, MESH: Insect Vectors/genetics, MESH: Insect Vectors/growth & development, MESH: Jordan, MESH: Phlebotomus/classification, MESH: Phlebotomus/genetics, MESH: Phlebotomus/growth & development, MESH: Phylogeography, MESH: Egypt, MESH: Genetic Variation, MESH: Genetics, Population, MESH: Haplotypes, [SDV]Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| Περιγραφή: | International audience ; Phlebotomus papatasi sand flies are major vectors of Leishmania major and phlebovirus infection in North Africa and across the Middle East to the Indian subcontinent. Population genetics is a valuable tool in understanding the level of genetic variability present in vector populations, vector competence, and the development of novel control strategies. This study investigated the genetic differentiation between P. papatasi populations in Egypt and Jordan that inhabit distinct ecotopes and compared this structure to P. papatasi populations from a broader geographical range.METHODS:A 461 base pair (bp) fragment from the mtDNA cytochrome b (cyt b) gene was PCR amplified and sequenced from 116 individual female sand flies from Aswan and North Sinai, Egypt, as well as Swaimeh and Malka, Jordan. Haplotypes were identified and used to generate a median-joining network, F ST values and isolation-by-distance were also evaluated. Additional sand fly individuals from Afghanistan, Iran, Israel, Jordan, Libya, Tunisia and Turkey were included as well as previously published haplotypes to provide a geographically broad genetic variation analysis.RESULTS:Thirteen haplotypes displaying nine variant sites were identified from P. papatasi collected in Egypt and Jordan. No private haplotypes were identified from samples in North Sinai, Egypt, two were observed in Aswan, Egypt, four from Swaimeh, Jordan and two in Malka, Jordan. The Jordan populations clustered separately from the Egypt populations and produced more private haplotypes than those from Egypt. Pairwise F ST values fall in the range 0.024-0.648.CONCLUSION:The clustering patterns and pairwise F ST values indicate a strong differentiation between Egyptian and Jordanian populations, although this population structure is not due to isolation-by-distance. Other factors, such as environmental influences and the genetic variability in the circulating Le. major parasites, could possibly contribute to this heterogeneity. The present study aligns with ... |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/29587873; PUBMED: 29587873; PUBMEDCENTRAL: PMC5872541 |
| DOI: | 10.1186/s13071-018-2785-9 |
| Διαθεσιμότητα: | https://riip.hal.science/pasteur-01953773 https://riip.hal.science/pasteur-01953773v1/document https://riip.hal.science/pasteur-01953773v1/file/flanley.pdf https://doi.org/10.1186/s13071-018-2785-9 |
| Rights: | https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Αριθμός Καταχώρησης: | edsbas.950654E9 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1186/s13071-018-2785-9 |
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