Academic Journal
Detection of a Mitochondrial Fragmentation and Integrated Stress Response Using the Cell Painting Assay
| Τίτλος: | Detection of a Mitochondrial Fragmentation and Integrated Stress Response Using the Cell Painting Assay |
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| Συγγραφείς: | Rezaei Adariani, Soheila, Agne, Daya, Koska, Sandra, Burhop, Annina, Seitz, Carina, Warmers, Jens, Janning, Petra, Metz, Malte, Pahl, Axel, Sievers, Sonja, Waldmann, Herbert, Ziegler, Slava |
| Πηγή: | http://lobid.org/resources/99370676049806441#!, 67(15):13252-13270. |
| Έτος έκδοσης: | 2024 |
| Συλλογή: | Publisso (ZB MED-Publikationsportal Lebenswissenschaften) |
| Θεματικοί όροι: | Small Molecule Libraries/chemistry [MeSH], Cell Line, Tumor [MeSH], Article, Stress, Physiological/drug effects [MeSH], Mitochondria/metabolism [MeSH], Mitochondria/drug effects [MeSH], Humans [MeSH], Small Molecule Libraries/pharmacology [MeSH] |
| Περιγραφή: | Mitochondria are cellular powerhouses and are crucial for cell function. However, they are vulnerable to internal and external perturbagens that may impair mitochondrial function and eventually lead to cell death. In particular, small molecules may impact mitochondrial function, and therefore, their influence on mitochondrial homeostasis is at best assessed early on in the characterization of biologically active small molecules and drug discovery. We demonstrate that unbiased morphological profiling by means of the cell painting assay (CPA) can detect mitochondrial stress coupled with the induction of an integrated stress response. This activity is common for compounds addressing different targets, is not shared by direct inhibitors of the electron transport chain, and enables prediction of mitochondrial stress induction for small molecules that are profiled using CPA. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | https://repository.publisso.de/resource/frl:6486328; https://doi.org/10.1021/acs.jmedchem.4c01183; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320566/ |
| DOI: | 10.1021/acs.jmedchem.4c01183 |
| Διαθεσιμότητα: | https://repository.publisso.de/resource/frl:6486328 https://doi.org/10.1021/acs.jmedchem.4c01183 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11320566/ |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Αριθμός Καταχώρησης: | edsbas.9071A515 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1021/acs.jmedchem.4c01183 |
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