Dataset
Data_Sheet_1_Selection of internal reference gene for normalization of reverse transcription-quantitative polymerase chain reaction analysis in Mycoplasma hyopneumoniae.docx
| Τίτλος: | Data_Sheet_1_Selection of internal reference gene for normalization of reverse transcription-quantitative polymerase chain reaction analysis in Mycoplasma hyopneumoniae.docx |
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| Συγγραφείς: | Shiyang Li, Yanqing Zhou, Ting Yuan, Zhixin Feng, Zhenzhen Zhang, Yuzi Wu, Qingyun Xie, Jia Wang, Quan Li, Zhibang Deng, Yanfei Yu, Xiaomin Yuan |
| Έτος έκδοσης: | 2022 |
| Συλλογή: | Frontiers: Figshare |
| Θεματικοί όροι: | Animal Systematics and Taxonomy, Animal Physiology - Biophysics, Animal Physiology - Cell, Animal Physiology - Systems, Animal Behaviour, Animal Cell and Molecular Biology, Animal Developmental and Reproductive Biology, Animal Immunology, Animal Neurobiology, Animal Physiological Ecology, Animal Structure and Function, Veterinary Anaesthesiology and Intensive Care, Veterinary Anatomy and Physiology, Veterinary Diagnosis and Diagnostics, Veterinary Epidemiology, Veterinary Immunology, Veterinary Medicine, Veterinary Microbiology (excl. Virology), Veterinary Parasitology, Veterinary Pathology, Veterinary Pharmacology, Veterinary Surgery, Veterinary Virology, Veterinary Sciences not elsewhere classified, Mycoplasma hyopneumoniae, RT-qPCR – real-time quantitative polymerase chain reaction, internal reference genes, gatB, virulence |
| Περιγραφή: | Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), which resulting in considerable economic losses in pig farming globally. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a major tool for gene expression studies. However, no internal reference genes for normalization of RT-qPCR data of M. hyopneumoniae have been reported. The aim of this study was to screen the most stable genes for RT-qPCR analysis in M. hyopneumoniae under different conditions. Therefore, a total of 13 candidate internal reference genes (rpoC, Lipo, sgaB, oppB, hypo621, oppF, gyrB, uvrA, P146, prfA, proS, gatB, and hypo499) of M. hyopneumoniae filtered according to the reported quantitative proteomic analysis and the 16S rRNA internal reference gene frequently used in other bacteria were selected for RT-qPCR analysis. The mRNAs from different virulence strains (168, 168 L, J, NJ, and LH) at five different growth phases were extracted. The corresponding cycle threshold (Ct) values of the 25 reverse transcribed cDNAs using the 14 candidate genes were determined. Different internal reference genes or combinations were then screened for expression stability analysis using various statistical tools and algorithms, including geNorm, BestKeeper, and NormFinder software, to ensure the reliability of the analysis. Through further comprehensive evaluation of the RefFinder software, it is concluded that the gatB gene was the most suitable internal reference gene for samples of the different virulence strains in different growth phases for M. hyopneumoniae, followed by prfA, hypo499, and gyrB. |
| Τύπος εγγράφου: | dataset |
| Γλώσσα: | unknown |
| DOI: | 10.3389/fvets.2022.934907.s001 |
| Διαθεσιμότητα: | https://doi.org/10.3389/fvets.2022.934907.s001 https://figshare.com/articles/dataset/Data_Sheet_1_Selection_of_internal_reference_gene_for_normalization_of_reverse_transcription-quantitative_polymerase_chain_reaction_analysis_in_Mycoplasma_hyopneumoniae_docx/20358201 |
| Rights: | CC BY 4.0 |
| Αριθμός Καταχώρησης: | edsbas.87762BBE |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.3389/fvets.2022.934907.s001 |
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