Academic Journal
View in EDS

Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis

Bibliographic Details
Title: Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis
Authors: Boulestreau, Jérémy, Maumus, Marie, Minani, Giuliana, Bertolino, Jorgensen, Christian, Noël, Danièle
Contributors: Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Service de Rhumatologie CHU de Montpellier, CHU Montpellier, FOREUM Foundation for Research in Rheumatology, ANR-22-PEBI-0013,STROMAEV,From engineering of iPS-derived mesenchymal stromal cell-derived extracellular vesicles to clinical translation(2022), ANR-22-AIBB-0007,OBBI,Occitanie Biotherapy-Bioproduction Integrator : Intégrateur de Bioproduction de Vésicules Extracellulaires et d'Organoïdes en Occitanie(2022), ANR-11-INBS-0005,ECELLFRANCE,Développement d'une Plateforme Nationale pour la médecine régénératrice(2011)
Source: EISSN: 1945-4589 ; Aging ; https://inserm.hal.science/inserm-04888483 ; Aging, 2024, 16 (21), pp.13252-13270. ⟨10.18632/aging.206158⟩
Publisher Information: CCSD
Impact Journals
Publication Year: 2024
Collection: Université de Montpellier: HAL
Subject Terms: osteoarthritis, aging, senescence, extracellular vesicle, mesenchymal stromal cell, regenerative medicine, MESH: Animals, MESH: Cells, Cultured, MESH: Mesenchymal Stem Cells* / metabolism, MESH: Mice, MESH: Osteoarthritis* / metabolism, MESH: Osteoarthritis* / therapy, MESH: Oxidative Stress / drug effects, MESH: Senescence-Associated Secretory Phenotype, MESH: Cellular Senescence, MESH: Chondrocytes* / metabolism, MESH: DNA Damage, MESH: Disease Models, Animal, MESH: Extracellular Vesicles* / metabolism, MESH: Extracellular Vesicles* / transplantation, MESH: Humans, MESH: Male, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
Description: International audience ; Age is the most important risk factor for degenerative diseases such as osteoarthritis (OA). It is associated with the accumulation of senescent cells in joint tissues that contribute to the pathogenesis of OA, in particular through the release of senescence-associated secretory phenotype (SASP) factors. Mesenchymal stromal cells (MSCs) and their derived extracellular vesicles (EVs) are promising treatments for OA. However, the senoprotective effects of MSC-derived EVs in OA have been poorly investigated. Here, we used EVs from human adipose tissue-derived MSCs (ASC-EVs) in two models of inflammaging (IL1β)-and DNA damage (etoposide)induced senescence in OA chondrocytes. We showed that the addition of ASC-EVs was effective in reducing senescence parameters, including the number of SA-β-Gal-positive cells, the accumulation of γH2AX foci in nuclei and the secretion of SASP factors. In addition, ASC-EVs demonstrated therapeutic efficacy when injected into a murine model of OA. Several markers of senescence, inflammation and oxidative stress were decreased shortly after injection likely explaining the therapeutic efficacy. In conclusion, ASC-EVs exert a senoprotective function both in vitro, in two models of induced senescence in OA chondrocytes and, in vivo, in the murine model of collagenase-induced OA.
Document Type: article in journal/newspaper
Language: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/39578049; PUBMED: 39578049; PUBMEDCENTRAL: PMC11719114
DOI: 10.18632/aging.206158
Availability: https://inserm.hal.science/inserm-04888483
https://inserm.hal.science/inserm-04888483v1/document
https://inserm.hal.science/inserm-04888483v1/file/2024-aging-J%C3%A9r%C3%A9my.pdf
https://doi.org/10.18632/aging.206158
Rights: http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
Accession Number: edsbas.83DC42DD
Database: BASE
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://inserm.hal.science/inserm-04888483#
    Name: EDS - BASE (ns324271)
    Category: fullText
    Text: View record from BASE
Header DbId: edsbas
DbLabel: BASE
An: edsbas.83DC42DD
RelevancyScore: 950
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 949.707946777344
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Boulestreau%2C+Jérémy%22">Boulestreau, Jérémy</searchLink><br /><searchLink fieldCode="AR" term="%22Maumus%2C+Marie%22">Maumus, Marie</searchLink><br /><searchLink fieldCode="AR" term="%22Minani%2C+Giuliana%2C+Bertolino%22">Minani, Giuliana, Bertolino</searchLink><br /><searchLink fieldCode="AR" term="%22Jorgensen%2C+Christian%22">Jorgensen, Christian</searchLink><br /><searchLink fieldCode="AR" term="%22Noël%2C+Danièle%22">Noël, Danièle</searchLink>
– Name: Author
  Label: Contributors
  Group: Au
  Data: Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)<br />Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)<br />Service de Rhumatologie CHU de Montpellier<br />CHU Montpellier<br />FOREUM Foundation for Research in Rheumatology<br />ANR-22-PEBI-0013,STROMAEV,From engineering of iPS-derived mesenchymal stromal cell-derived extracellular vesicles to clinical translation(2022)<br />ANR-22-AIBB-0007,OBBI,Occitanie Biotherapy-Bioproduction Integrator : Intégrateur de Bioproduction de Vésicules Extracellulaires et d'Organoïdes en Occitanie(2022)<br />ANR-11-INBS-0005,ECELLFRANCE,Développement d'une Plateforme Nationale pour la médecine régénératrice(2011)
– Name: TitleSource
  Label: Source
  Group: Src
  Data: EISSN: 1945-4589 ; Aging ; https://inserm.hal.science/inserm-04888483 ; Aging, 2024, 16 (21), pp.13252-13270. ⟨10.18632/aging.206158⟩
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: CCSD<br />Impact Journals
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2024
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: Université de Montpellier: HAL
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22osteoarthritis%22">osteoarthritis</searchLink><br /><searchLink fieldCode="DE" term="%22aging%22">aging</searchLink><br /><searchLink fieldCode="DE" term="%22senescence%22">senescence</searchLink><br /><searchLink fieldCode="DE" term="%22extracellular+vesicle%22">extracellular vesicle</searchLink><br /><searchLink fieldCode="DE" term="%22mesenchymal+stromal+cell%22">mesenchymal stromal cell</searchLink><br /><searchLink fieldCode="DE" term="%22regenerative+medicine%22">regenerative medicine</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Animals%22">MESH: Animals</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Cells%22">MESH: Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Cultured%22">Cultured</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Mesenchymal+Stem+Cells*+%2F+metabolism%22">MESH: Mesenchymal Stem Cells* / metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Mice%22">MESH: Mice</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Osteoarthritis*+%2F+metabolism%22">MESH: Osteoarthritis* / metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Osteoarthritis*+%2F+therapy%22">MESH: Osteoarthritis* / therapy</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Oxidative+Stress+%2F+drug+effects%22">MESH: Oxidative Stress / drug effects</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Senescence-Associated+Secretory+Phenotype%22">MESH: Senescence-Associated Secretory Phenotype</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Cellular+Senescence%22">MESH: Cellular Senescence</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Chondrocytes*+%2F+metabolism%22">MESH: Chondrocytes* / metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+DNA+Damage%22">MESH: DNA Damage</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Disease+Models%22">MESH: Disease Models</searchLink><br /><searchLink fieldCode="DE" term="%22Animal%22">Animal</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Extracellular+Vesicles*+%2F+metabolism%22">MESH: Extracellular Vesicles* / metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Extracellular+Vesicles*+%2F+transplantation%22">MESH: Extracellular Vesicles* / transplantation</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Humans%22">MESH: Humans</searchLink><br /><searchLink fieldCode="DE" term="%22MESH%3A+Male%22">MESH: Male</searchLink><br /><searchLink fieldCode="DE" term="%22[SDV%2EMHEP%2ERSOA]Life+Sciences+[q-bio]%2FHuman+health+and+pathology%2FRhumatology+and+musculoskeletal+system%22">[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: International audience ; Age is the most important risk factor for degenerative diseases such as osteoarthritis (OA). It is associated with the accumulation of senescent cells in joint tissues that contribute to the pathogenesis of OA, in particular through the release of senescence-associated secretory phenotype (SASP) factors. Mesenchymal stromal cells (MSCs) and their derived extracellular vesicles (EVs) are promising treatments for OA. However, the senoprotective effects of MSC-derived EVs in OA have been poorly investigated. Here, we used EVs from human adipose tissue-derived MSCs (ASC-EVs) in two models of inflammaging (IL1β)-and DNA damage (etoposide)induced senescence in OA chondrocytes. We showed that the addition of ASC-EVs was effective in reducing senescence parameters, including the number of SA-β-Gal-positive cells, the accumulation of γH2AX foci in nuclei and the secretion of SASP factors. In addition, ASC-EVs demonstrated therapeutic efficacy when injected into a murine model of OA. Several markers of senescence, inflammation and oxidative stress were decreased shortly after injection likely explaining the therapeutic efficacy. In conclusion, ASC-EVs exert a senoprotective function both in vitro, in two models of induced senescence in OA chondrocytes and, in vivo, in the murine model of collagenase-induced OA.
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article in journal/newspaper
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: info:eu-repo/semantics/altIdentifier/pmid/39578049; PUBMED: 39578049; PUBMEDCENTRAL: PMC11719114
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.18632/aging.206158
– Name: URL
  Label: Availability
  Group: URL
  Data: https://inserm.hal.science/inserm-04888483<br />https://inserm.hal.science/inserm-04888483v1/document<br />https://inserm.hal.science/inserm-04888483v1/file/2024-aging-J%C3%A9r%C3%A9my.pdf<br />https://doi.org/10.18632/aging.206158
– Name: Copyright
  Label: Rights
  Group: Cpyrght
  Data: http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsbas.83DC42DD
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsbas&AN=edsbas.83DC42DD
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.18632/aging.206158
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: osteoarthritis
        Type: general
      – SubjectFull: aging
        Type: general
      – SubjectFull: senescence
        Type: general
      – SubjectFull: extracellular vesicle
        Type: general
      – SubjectFull: mesenchymal stromal cell
        Type: general
      – SubjectFull: regenerative medicine
        Type: general
      – SubjectFull: MESH: Animals
        Type: general
      – SubjectFull: MESH: Cells
        Type: general
      – SubjectFull: Cultured
        Type: general
      – SubjectFull: MESH: Mesenchymal Stem Cells* / metabolism
        Type: general
      – SubjectFull: MESH: Mice
        Type: general
      – SubjectFull: MESH: Osteoarthritis* / metabolism
        Type: general
      – SubjectFull: MESH: Osteoarthritis* / therapy
        Type: general
      – SubjectFull: MESH: Oxidative Stress / drug effects
        Type: general
      – SubjectFull: MESH: Senescence-Associated Secretory Phenotype
        Type: general
      – SubjectFull: MESH: Cellular Senescence
        Type: general
      – SubjectFull: MESH: Chondrocytes* / metabolism
        Type: general
      – SubjectFull: MESH: DNA Damage
        Type: general
      – SubjectFull: MESH: Disease Models
        Type: general
      – SubjectFull: Animal
        Type: general
      – SubjectFull: MESH: Extracellular Vesicles* / metabolism
        Type: general
      – SubjectFull: MESH: Extracellular Vesicles* / transplantation
        Type: general
      – SubjectFull: MESH: Humans
        Type: general
      – SubjectFull: MESH: Male
        Type: general
      – SubjectFull: [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
        Type: general
    Titles:
      – TitleFull: Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Boulestreau, Jérémy
      – PersonEntity:
          Name:
            NameFull: Maumus, Marie
      – PersonEntity:
          Name:
            NameFull: Minani, Giuliana, Bertolino
      – PersonEntity:
          Name:
            NameFull: Jorgensen, Christian
      – PersonEntity:
          Name:
            NameFull: Noël, Danièle
      – PersonEntity:
          Name:
            NameFull: Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
      – PersonEntity:
          Name:
            NameFull: Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)
      – PersonEntity:
          Name:
            NameFull: Service de Rhumatologie CHU de Montpellier
      – PersonEntity:
          Name:
            NameFull: CHU Montpellier
      – PersonEntity:
          Name:
            NameFull: FOREUM Foundation for Research in Rheumatology
      – PersonEntity:
          Name:
            NameFull: ANR-22-PEBI-0013,STROMAEV,From engineering of iPS-derived mesenchymal stromal cell-derived extracellular vesicles to clinical translation(2022)
      – PersonEntity:
          Name:
            NameFull: ANR-22-AIBB-0007,OBBI,Occitanie Biotherapy-Bioproduction Integrator : Intégrateur de Bioproduction de Vésicules Extracellulaires et d'Organoïdes en Occitanie(2022)
      – PersonEntity:
          Name:
            NameFull: ANR-11-INBS-0005,ECELLFRANCE,Développement d'une Plateforme Nationale pour la médecine régénératrice(2011)
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 01
              Type: published
              Y: 2024
          Identifiers:
            – Type: issn-locals
              Value: edsbas
            – Type: issn-locals
              Value: edsbas.oa
          Titles:
            – TitleFull: EISSN: 1945-4589 ; Aging ; https://inserm.hal.science/inserm-04888483 ; Aging, 2024, 16 (21), pp.13252-13270. ⟨10.18632/aging.206158⟩
              Type: main
ResultId 1