Academic Journal
Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis
| Τίτλος: | Anti-aging effect of extracellular vesicles from mesenchymal stromal cells on senescence-induced chondrocytes in osteoarthritis |
|---|---|
| Συγγραφείς: | Boulestreau, Jérémy, Maumus, Marie, Minani, Giuliana, Bertolino, Jorgensen, Christian, Noël, Danièle |
| Συνεισφορές: | Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Service de Rhumatologie CHU de Montpellier, CHU Montpellier, FOREUM Foundation for Research in Rheumatology, ANR-22-PEBI-0013,STROMAEV,From engineering of iPS-derived mesenchymal stromal cell-derived extracellular vesicles to clinical translation(2022), ANR-22-AIBB-0007,OBBI,Occitanie Biotherapy-Bioproduction Integrator : Intégrateur de Bioproduction de Vésicules Extracellulaires et d'Organoïdes en Occitanie(2022), ANR-11-INBS-0005,ECELLFRANCE,Développement d'une Plateforme Nationale pour la médecine régénératrice(2011) |
| Πηγή: | EISSN: 1945-4589 ; Aging ; https://inserm.hal.science/inserm-04888483 ; Aging, 2024, 16 (21), pp.13252-13270. ⟨10.18632/aging.206158⟩ |
| Στοιχεία εκδότη: | CCSD Impact Journals |
| Έτος έκδοσης: | 2024 |
| Συλλογή: | Université de Montpellier: HAL |
| Θεματικοί όροι: | osteoarthritis, aging, senescence, extracellular vesicle, mesenchymal stromal cell, regenerative medicine, MESH: Animals, MESH: Cells, Cultured, MESH: Mesenchymal Stem Cells* / metabolism, MESH: Mice, MESH: Osteoarthritis* / metabolism, MESH: Osteoarthritis* / therapy, MESH: Oxidative Stress / drug effects, MESH: Senescence-Associated Secretory Phenotype, MESH: Cellular Senescence, MESH: Chondrocytes* / metabolism, MESH: DNA Damage, MESH: Disease Models, Animal, MESH: Extracellular Vesicles* / metabolism, MESH: Extracellular Vesicles* / transplantation, MESH: Humans, MESH: Male, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system |
| Περιγραφή: | International audience ; Age is the most important risk factor for degenerative diseases such as osteoarthritis (OA). It is associated with the accumulation of senescent cells in joint tissues that contribute to the pathogenesis of OA, in particular through the release of senescence-associated secretory phenotype (SASP) factors. Mesenchymal stromal cells (MSCs) and their derived extracellular vesicles (EVs) are promising treatments for OA. However, the senoprotective effects of MSC-derived EVs in OA have been poorly investigated. Here, we used EVs from human adipose tissue-derived MSCs (ASC-EVs) in two models of inflammaging (IL1β)-and DNA damage (etoposide)induced senescence in OA chondrocytes. We showed that the addition of ASC-EVs was effective in reducing senescence parameters, including the number of SA-β-Gal-positive cells, the accumulation of γH2AX foci in nuclei and the secretion of SASP factors. In addition, ASC-EVs demonstrated therapeutic efficacy when injected into a murine model of OA. Several markers of senescence, inflammation and oxidative stress were decreased shortly after injection likely explaining the therapeutic efficacy. In conclusion, ASC-EVs exert a senoprotective function both in vitro, in two models of induced senescence in OA chondrocytes and, in vivo, in the murine model of collagenase-induced OA. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/39578049; PUBMED: 39578049; PUBMEDCENTRAL: PMC11719114 |
| DOI: | 10.18632/aging.206158 |
| Διαθεσιμότητα: | https://inserm.hal.science/inserm-04888483 https://inserm.hal.science/inserm-04888483v1/document https://inserm.hal.science/inserm-04888483v1/file/2024-aging-J%C3%A9r%C3%A9my.pdf https://doi.org/10.18632/aging.206158 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Αριθμός Καταχώρησης: | edsbas.83DC42DD |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.18632/aging.206158 |
|---|