Academic Journal
Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant
| Τίτλος: | Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant |
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| Συγγραφείς: | Mlcochova P., Collier D., Ritchie A., Assennato S. M., Hosmillo M., Goel N., Meng B., Chatterjee K., Mendoza V., Temperton N., Kiss L., James L. C., Ciazynska K. A., Xiong X., Briggs J. A. G., Nathan J. A., Mescia F., Bergamaschi L., Zhang H., Barmpounakis P., Demeris N., Skells R., Lyons P. A., Bradley J., Baker S., Allain J. P., Smith K. G. C., Bousfield R., Wilson M., Sparkes D., Amoroso G., Gkrania-Klotsas E., Hardwick S., Boyle A., Goodfellow I., Gupta R. K., Dougan G., Gupta R., Lehner P. J., Lyons P., Matheson N. J., Toshner M., Weekes M. P., Brown N., Curran M., Palmar S., Enoch D., Chapman D., Shaw A., Jose S., Bermperi A., Zerrudo J. A., Kourampa E., Watson L., Worsley J., Saunders C., de Jesus R., Domingo J., Pasquale C., Vergese B., Vargas P., Fabiculana M., Perales M., Mynott L., Blake E., Bates A., Vallier A. -L., Williams A., Phillips D., Chiu E., Overhill A., Ramenatte N., Sipple J., Frost S., Knock H., Hardy R., Foster E., Davidson F., Rundell V., Bundi P., Abeseabe R., Clark S., Vicente I., Elmer A., Ribeiro C., Kourampa J., Kennet J., Rowlands J., Meadows A., O'Brien C., Rastall R., Crucusio C., Hewitt S., Price J., Calder J., Canna L., Bucke A., Tordesillas H., Harris J., Ruffolo V., Graves B., Butcher H., Caputo D., Le Gresley E., Dunmore B. J., Martin J., Legchenko E., Treacy C., Huang C., Wood J., Sutcliffe R., Hodgson J., Shih J., Graf S., Tong Z., Tilly T., O'Donnell C., Hunter K., Pointon L., Pond N., Wylot M., Jones E., Fawke S., Bullman B., Turner L., Jarvis I., Omarjee O., De Sa A., Marsden J., Betancourt A., Perera M., Epping M., Richoz N., Bower G., Sharma R., Nice F., Huhn O., Savoinykh Yarkoni N., Romashova N., Lewis D., Hinch A., Cossetti C., Strezlecki M., Grenfell R., Stark H., Walker N., Stirrups K., Ovington N., Dewhust E., Li E., Papadia S., Kingston N., Lever A., Torok E., Hamilton W., Hall G., Jahun A., Chaudhry Y., Pinckert M., Georgana I., Yakovleva A., Caller L., Caddy S., Feltwell T., Khokhar F., Meredith L., Holdcroft C., Parmar S. |
| Συνεισφορές: | Mlcochova P., Collier D., Ritchie A., Assennato S. M., Hosmillo M., Goel N., Meng B., Chatterjee K., Mendoza V., Temperton N., Kiss L., James L. C., Ciazynska K. A., Xiong X., Briggs J. A. G., Nathan J. A., Mescia F., Bergamaschi L., Zhang H., Barmpounakis P., Demeris N., Skells R., Lyons P. A., Bradley J., Baker S., Allain J. P., Smith K. G. C., Bousfield R., Wilson M., Sparkes D., Amoroso G., Gkrania-Klotsas E., Hardwick S., Boyle A., Goodfellow I., Gupta R. K., Dougan G., Gupta R., Lehner P. J., Lyons P., Matheson N. J., Toshner M., Weekes M. P., Brown N., Curran M., Palmar S., Enoch D., Chapman D., Shaw A., Jose S., Bermperi A., Zerrudo J. A., Kourampa E., Watson L., Worsley J., Saunders C., de Jesus R., Domingo J., Pasquale C., Vergese B., Vargas P., Fabiculana M., Perales M., Mynott L., Blake E., Bates A., Vallier A. -L., Williams A., Phillips D., Chiu E., Overhill A., Ramenatte N., Sipple J., Frost S., Knock H., Hardy R., Foster E., Davidson F., Rundell V., Bundi P., Abeseabe R., Clark S., Vicente I., Elmer A., Ribeiro C., Kourampa J., Kennet J., Rowlands J., Meadows A., O'Brien C., Rastall R., Crucusio C., Hewitt S., Price J., Calder J., Canna L., Bucke A., Tordesillas H., Harris J. |
| Έτος έκδοσης: | 2020 |
| Συλλογή: | Università degli Studi di Brescia: OPENBS - Open Archive UniBS |
| Θεματικοί όροι: | COVID-19, D614G, point of care testing, rapid diagnose, SARS-CoV-2, Aged, 80 and over, Antibodies, Viral, COVID-19 Testing, Female, Human, Immunoassay, Male, Middle Aged, Neutralization Test, Nucleic Acid Amplification Technique, Sensitivity and Specificity, Spike Glycoprotein, Coronaviru, Point-of-Care Testing |
| Περιγραφή: | Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%–50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wild-type or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8–92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32905045; info:eu-repo/semantics/altIdentifier/wos/WOS:000642300100009; volume:1; issue:6; firstpage:100099; journal:CELL REPORTS MEDICINE; https://hdl.handle.net/11379/562819 |
| DOI: | 10.1016/j.xcrm.2020.100099 |
| Διαθεσιμότητα: | https://hdl.handle.net/11379/562819 https://doi.org/10.1016/j.xcrm.2020.100099 |
| Rights: | info:eu-repo/semantics/openAccess ; license:PUBBLICO - Creative Commons 4.0 ; license uri:iris.PUB15 |
| Αριθμός Καταχώρησης: | edsbas.7B9074E4 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1016/j.xcrm.2020.100099 |
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