Academic Journal
Immunization of BALB/c mice with Helicobacter urease B induces a T helper 2 response absent in Helicobacter infection.
| Title: | Immunization of BALB/c mice with Helicobacter urease B induces a T helper 2 response absent in Helicobacter infection. |
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| Authors: | Saldinger, P.F., Porta, N., Launois, P., Louis, J.A., Waanders, G.A., Bouzouréne, H., Michetti, P., Blum, A.L., Corthésy-Theulaz, I.E. |
| Publication Year: | 1998 |
| Collection: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Subject Terms: | Animals, Antibody Formation/immunology, Cell Division/drug effects, Cholera Toxin/immunology, Cytokines/metabolism, Female, Gastric Mucosa/immunology, Gastric Mucosa/metabolism, Helicobacter/enzymology, Helicobacter Infections/immunology, Helicobacter Infections/therapy, Immunization, Isoenzymes/immunology, Mice, Inbred BALB C, Recombinant Proteins, T-Lymphocytes/pathology, T-Lymphocytes, Helper-Inducer/immunology, Th1 Cells/pathology, Th2 Cells/pathology, Urease/immunology |
| Description: | BACKGROUND & AIMS: Infection with Helicobacter induces a T helper type 1 response in mice and humans. Mice can be cured or protected from infection with Helicobacter by mucosal immunization with recombinant H. pylori urease B subunit (rUreB). This study characterizes the immune response of infected mice immunized with rUreB. METHODS: BALB/c mice were infected with H. felis. Two weeks later, they were orally immunized four times with rUreB and cholera toxin (CT) at weekly intervals. Controls were only infected or sham-immunized with CT. Animals were killed at various times after immunization. Splenic CD4(+) cells were obtained and cultured in vitro with rUreB to evaluate antigen-specific proliferation and induction of interferon gamma and interleukin 4 secretion. RESULTS: All rUreB-immunized mice (n = 8) were cured from infection 3 weeks after the fourth immunization. Immunization induced a proliferative response of splenic CD4(+) cells, a progressive decrease in interferon gamma secretion, and a concomitant increase in interleukin 4 secretion after each immunization. A simultaneous increase in rUreB specific serum immunoglobulin G1 levels was observed in infected/immunized mice. CONCLUSIONS: In BALB/c mice, therapeutic mucosal immunization with rUreB induces progressively a Th2 CD4(+) T cell response resulting in the elimination of the pathogen. |
| Document Type: | article in journal/newspaper |
| Language: | English |
| ISSN: | 0016-5085 |
| Relation: | Gastroenterology; https://iris.unil.ch/handle/iris/162031; serval:BIB_C423624C1CA2; 000076136500016; 9753492 |
| DOI: | 10.1016/S0016-5085(98)70261-6 |
| Availability: | https://iris.unil.ch/handle/iris/162031 https://doi.org/10.1016/S0016-5085(98)70261-6 |
| Accession Number: | edsbas.7A45B82E |
| Database: | BASE |
| ISSN: | 00165085 |
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| DOI: | 10.1016/S0016-5085(98)70261-6 |