Academic Journal
Performance of the Xpert HBV Viral Load assay versus the Aptima Quant assay for quantifying hepatitis B virus DNA
| Τίτλος: | Performance of the Xpert HBV Viral Load assay versus the Aptima Quant assay for quantifying hepatitis B virus DNA |
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| Συγγραφείς: | Abravanel, Florence, Lhomme, Sébastien, Trémeaux, Pauline, Migueres, Marion, Harter, Agnès, Haslé, Catherine, Bruel, Patrick, Alric, Laurent, Métivier, Sophie, Raymond, Stéphanie, Izopet, Jacques |
| Συνεισφορές: | Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Communauté d'universités et établissements de Toulouse (Comue de Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Virologie CHU Toulouse, Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie CHU Toulouse, Centre Hospitalier Universitaire de Toulouse (CHU Toulouse) |
| Πηγή: | ISSN: 0732-8893. |
| Στοιχεία εκδότη: | CCSD Elsevier |
| Έτος έκδοσης: | 2020 |
| Συλλογή: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| Θεματικοί όροι: | Molecular diagnosis, Hepatitis B virus, GeneXpert instrument, MESH: DNA, Viral, MESH: Genotype, MESH: Sensitivity and Specificity, MESH: Viral Load / methods, MESH: Viral Load / standards, MESH: Workflow, MESH: Hepatitis B / diagnosis, MESH: Hepatitis B / virology, MESH: Hepatitis B virus / genetics, MESH: Humans, MESH: Molecular Diagnostic Techniques, MESH: Reproducibility of Results, [SDV]Life Sciences [q-bio] |
| Περιγραφή: | International audience ; Quantification of HBV DNA is used for initiating and monitoring antiviral treatment. We have evaluated the Xpert HBV Viral Load (VL) assay on the GeneXpert instrument. We estimated its limit of detection to be 7.5 IU/mL. Reproducibility was 1.1-12.7% as assessed by the coefficients of variation for 3 different samples. The assay was linear from 2 to 8 log10 IU/mL for HBV genotypes A to F. Its clinical performance was evaluated by testing prospectively 100 HBV DNA-positive samples with the Xpert HBV VL and Aptima Quant HBV assays. The results from the 2 assays were correlated, with a modest bias (-0.10 log10 IU/mL) between them by Bland-Altman analysis. Patient monitoring with 80 samples performed with both assays gave similar patient profiles with trends in the same direction. The Xpert HBV Viral load assay is reliable enough for quantifying HBV DNA in clinical practice. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/31771903; PUBMED: 31771903 |
| DOI: | 10.1016/j.diagmicrobio.2019.114946 |
| Διαθεσιμότητα: | https://hal.science/hal-03489830 https://hal.science/hal-03489830v1/document https://hal.science/hal-03489830v1/file/S0732889319307679.pdf https://doi.org/10.1016/j.diagmicrobio.2019.114946 |
| Rights: | http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| Αριθμός Καταχώρησης: | edsbas.79E383AA |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1016/j.diagmicrobio.2019.114946 |
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