Academic Journal
Tertiary lymphoid structure-related immune infiltrates in NSCLC tumor lesions correlate with low tumor-reactivity of TIL products.
| Τίτλος: | Tertiary lymphoid structure-related immune infiltrates in NSCLC tumor lesions correlate with low tumor-reactivity of TIL products. |
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| Συγγραφείς: | Castenmiller, S.M., Kanagasabesan, N., Guislain, A., Nicolet, B.P., van Loenen, M.M., Monkhorst, K., Veenhof, AAFA, Smit, E.F., Hartemink, K.J., Haanen, JBAG, de Groot, R., Wolkers, M.C. |
| Έτος έκδοσης: | 2024 |
| Συλλογή: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Θεματικοί όροι: | Humans, Carcinoma, Non-Small-Cell Lung/immunology, Non-Small-Cell Lung/pathology, Lung Neoplasms/immunology, Lung Neoplasms/pathology, Tertiary Lymphoid Structures/immunology, Tertiary Lymphoid Structures/pathology, Lymphocytes, Tumor-Infiltrating/immunology, Immunotherapy, Adoptive/methods, Female, Male, B-Lymphocytes/immunology, B-Lymphocytes/pathology, Middle Aged, Aged, Immune infiltrate, NSCLC, TIL therapy, TLS formation |
| Περιγραφή: | Adoptive transfer of tumor infiltrating lymphocytes (TIL therapy) has proven highly effective for treating solid cancers, including non-small cell lung cancer (NSCLC). However, not all patients benefit from this therapy for yet unknown reasons. Defining markers that correlate with high tumor-reactivity of the autologous TIL products is thus key for achieving better tailored immunotherapies. We questioned whether the composition of immune cell infiltrates correlated with the tumor-reactivity of expanded TIL products. Unbiased flow cytometry analysis of immune cell infiltrates of 26 early-stage and 20 late-stage NSCLC tumor lesions was used for correlations with the T cell differentiation and activation status, and with the expansion rate and anti-tumor response of generated TIL products. The composition of tumor immune infiltrates was highly variable between patients. Spearman's Rank Correlation revealed that high B cell infiltration negatively correlated with the tumor-reactivity of the patient's expanded TIL products, as defined by cytokine production upon exposure to autologous tumor digest. In-depth analysis revealed that tumor lesions with high B cell infiltrates contained tertiary lymphoid structure (TLS)-related immune infiltrates, including BCL6 + antibody-secreting B cells, IgD + BCL6 + B cells and CXCR5 + BLC6 + CD4 + T cells, and higher percentages of naïve CD8 + T cells. In conclusion, the composition of immune cell infiltrates in NSCLC tumors associates with the functionality of the expanded TIL product. Our findings may thus help improve patient selection for TIL therapy. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 2162-402X |
| Relation: | OncoImmunology; https://iris.unil.ch/handle/iris/50545; serval:BIB_4F0F8DC467FB; 001296385300001 |
| DOI: | 10.1080/2162402X.2024.2392898 |
| Διαθεσιμότητα: | https://iris.unil.ch/handle/iris/50545 https://doi.org/10.1080/2162402X.2024.2392898 |
| Αριθμός Καταχώρησης: | edsbas.78237934 |
| Βάση Δεδομένων: | BASE |
| ISSN: | 2162402X |
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| DOI: | 10.1080/2162402X.2024.2392898 |