Academic Journal
Limited passage and functional effects of polystyrene micro- and nanoplastics in a physiologically-relevant in vitro human placental co-culture model
| Τίτλος: | Limited passage and functional effects of polystyrene micro- and nanoplastics in a physiologically-relevant in vitro human placental co-culture model |
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| Συγγραφείς: | van Boxel, Jeske, Nijmeijer, Sandra M., Heinzelmann, Manuel T., Rupp, Sebastian, van Duursen, Majorie B.M. |
| Πηγή: | van Boxel, J, Nijmeijer, S M, Heinzelmann, M T, Rupp, S & van Duursen, M B M 2025, 'Limited passage and functional effects of polystyrene micro- and nanoplastics in a physiologically-relevant in vitro human placental co-culture model', Reproductive Toxicology, vol. 136, 108956, pp. 1-19. https://doi.org/10.1016/j.reprotox.2025.108956 |
| Έτος έκδοσης: | 2025 |
| Θεματικοί όροι: | Humans, Polystyrenes/toxicity, Coculture Techniques, Female, Placenta/drug effects, Pregnancy, Microplastics/toxicity, Human Umbilical Vein Endothelial Cells, Nanoparticles/toxicity, Oxidative Stress/drug effects |
| Περιγραφή: | The placenta plays a crucial role during pregnancy, yet effective in vitro models for placental toxicity testing are limited. In this study, a Transwell co-culture model combining BeWo b30 and HUVEC cells was developed and characterized, and used to study the transport and effects of polystyrene micro- and nanoplastics (PS-MNPs). After 72 h, 8.7 % of 50 nm and 1.2 % of 200 nm of fluorescent (F)PS-MNPs were detected on the basolateral side, while 1000 nm FPS-MNPs were undetectable. Confocal microscopy showed the uptake of 50 and 200 nm FPS-MNPs by the BeWo b30 and HUVEC cell layer, whereas the 1000 nm FPS-MNPs were only found within the BeWo b30 cell layer. Exposure to PS-MNPs (sizes of 50, 200 and 1000 nm at concentrations of 1-10 µg/mL) did not result in an effect on mitochondrial activity, oxidative stress and gene expression of several functional markers and steroidogenic enzymes. However, LC/MS-MS analysis of the culture media showed a decrease of 17 % in the level of 17-alpha-estradiol after 72-hour exposure to 1 µg/mL 50 nm PS-MNPs compared to vehicle control. Overall, our data showed limited effects of PS-MNPs on placental cell function in vitro, but FPS-MNPs were internalized and detected on the basolateral side in the co-culture. This warrants further studies on effects of MNPs on placental cell function, and particularly steroidogenesis, to assess the potential effects of MNPs during pregnancy. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| ISSN: | 0890-6238 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/40484253; info:eu-repo/semantics/altIdentifier/hdl/https://hdl.handle.net/1871.1/bfdaccd7-a267-4607-8c3b-9a08b7b848ef; info:eu-repo/semantics/altIdentifier/pissn/0890-6238 |
| DOI: | 10.1016/j.reprotox.2025.108956 |
| Διαθεσιμότητα: | https://research.vu.nl/en/publications/bfdaccd7-a267-4607-8c3b-9a08b7b848ef https://doi.org/10.1016/j.reprotox.2025.108956 https://hdl.handle.net/1871.1/bfdaccd7-a267-4607-8c3b-9a08b7b848ef https://www.scopus.com/pages/publications/105007657868 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Αριθμός Καταχώρησης: | edsbas.6E39A147 |
| Βάση Δεδομένων: | BASE |
| ISSN: | 08906238 |
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| DOI: | 10.1016/j.reprotox.2025.108956 |