Academic Journal
Distinct PlzC mechanisms integrate chemotaxis and c-di-GMP signaling to regulate Vibrio cholerae motility and biofilm formation
| Τίτλος: | Distinct PlzC mechanisms integrate chemotaxis and c-di-GMP signaling to regulate Vibrio cholerae motility and biofilm formation |
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| Συγγραφείς: | Park, Jin Hwan, Kennedy, Emily N., Tripathi, Sarvind, Romp, Avery B., Rubin, Seth M., Bourret, Robert B., Yildiz, Fitnat H. |
| Πηγή: | Proceedings of the National Academy of Sciences of the United States of America, 123(2) |
| Στοιχεία εκδότη: | National Academy of Sciences |
| Έτος έκδοσης: | 2026 |
| Συλλογή: | Carolina Digital Repository (UNC - University of North Carolina) |
| Θεματικοί όροι: | Phase transitions, Full-length 16S rRNA amplicon sequencing, Servicios de Salud Dental, renal replacement therapy, Organic crystals, leptomeningeal carcinomatosis, health services research, Emergency department, SRHR, Xpert® MTB/RIF ultra, aging, Resource-limited, Skeletal muscle, adaptive treatment assignment, Health, Anti-inflammatory activity, Meio Ambiente e Saúde Pública, neural dynamics model, Chronic disease, liver transplantation, prognostic factors, gastric biopsy, preference for dying at home, incidence, Venetoclax, dopamine receptor D2 (DRD2), 675 nm laser, pathogenesis, Youth, group dynamics |
| Περιγραφή: | Significance Bacterial motility and biofilm formation critically facilitate adaptation and survival and are regulated by the second messenger c-di-GMP. Vibrio cholerae PilZ domain proteins are c-di-GMP effectors, but the mechanisms by which they govern cellular processes are poorly understood. This study identifies PlzC as a critical integrator of motility and biofilm development via multiple pathways. PlzC promotes motility by regulating CheX, a CheY-3 phosphatase that impacts migration direction. While CheX modulates directional changes through CheY-3 dephosphorylation, PlzC more broadly impacts biofilm maturation, indicating PlzC has a dual regulatory role that targets different stages leading to biofilm formation. PlzC is a key link between chemotaxis and c-di-GMP signaling, providing insight into bacterial adaptability and potentially serving as a target for therapeutic approaches. ; Bacterial motility and biofilm formation are essential for the adaptation and survival of Vibrio cholerae, the causative agent of cholera. In many bacterial species, the second messenger c-di-GMP regulates these processes through PilZ domain proteins, however the downstream mechanisms have remained poorly defined. Here, we identify the PilZ domain protein PlzC as a key positive regulator of motility and biofilm formation through distinct mechanisms. A suppressor screen for mutants restoring ΔplzC migration identified downstream regulators, including CheX, a CheY-3 phosphatase. Genetic and phenotypic analyses revealed that PlzC promotes motility by modulating CheX, thereby influencing the frequency of direction changes during swimming. Notably, the role of PlzC in motility regulation is independent of CheZ, another CheY-3 phosphatase, demonstrating that among the two CheY-3 phosphatases, only CheX is under PlzC control. This distinction suggests that CheY-3-P levels are regulated by at least two separate signaling pathways, one of which operates through PlzC. Despite its function in motility, CheX is not required for ... |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | unknown |
| Relation: | https://cdr.lib.unc.edu/downloads/k0698r282?file=thumbnail; https://cdr.lib.unc.edu/downloads/k0698r282 |
| DOI: | 10.17615/t3jb-g473 |
| Διαθεσιμότητα: | https://doi.org/10.17615/t3jb-g473 https://cdr.lib.unc.edu/downloads/k0698r282?file=thumbnail https://cdr.lib.unc.edu/downloads/k0698r282 |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Αριθμός Καταχώρησης: | edsbas.4AFA4419 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.17615/t3jb-g473 |
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