Academic Journal
Internal controls for quantitative RT-PCR analysis of gene expression in response to ocean acidification in edible oysters
| Τίτλος: | Internal controls for quantitative RT-PCR analysis of gene expression in response to ocean acidification in edible oysters |
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| Συγγραφείς: | Dang, X, Noor, Z, He, YQ, Lim, YK, Zhang, Y, Yu, ZN, Thiyagarajan, V |
| Στοιχεία εκδότη: | Elsevier |
| Έτος έκδοσης: | 2022 |
| Συλλογή: | University of Hong Kong: HKU Scholars Hub |
| Θεματικοί όροι: | Crassostrea hongkongensis, Development, Internal reference genes, Ocean acidification, Real-time PCR |
| Περιγραφή: | The increase of CO2 by anthropogenic activities leads to a decrease of pH in the ocean surface due to ocean acidification (OA) process. Generally, OA not only reduces the rate of calcification in marine environments but also affects various physiological activities, especially in calcifiers, including edible oysters. Quantitative real-time PCR (qRT-PCR) is often used to detect gene expression in response to OA, which relies on the stability of internal control. However, the appropriate internal controls for OA experiments remain scarce especially in the marine calcifiers. Hence, this study developed internal controls for qRT-PCR assays using the Hong Kong oyster (Crassostrea hongkongensis) as a model to reveal gene expression profile during development under OA. In this study, 17 housekeeping genes were selected as the possible candidate of the internal controls. After a comprehensive interpretation from the multiple algorithms and software, GAPDH paired with RL23 is recommended for the normalization for planktonic larvae and benthic juveniles, but beyond that, TUBB and EF2 are recommended for post-metamorphic stage. Moreover, GAPDH and EF2 were suitable for various pH treatments, and TUBB, RL35 and RL23 could be the alternatives for OA experiments. These results are instrumental for the selection of internal control in Crassostrea hongkongensis during the development, and shed light on other molecular OA experiments in marine invertebrates for reference. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | Journal of Experimental Marine Biology and Ecology; Journal of Experimental Marine Biology and Ecology, 2022, v. 548; WOS:000788160100007; https://hub.hku.hk/handle/10722/338688; 548 |
| DOI: | 10.1016/j.jembe.2021.151683 |
| Διαθεσιμότητα: | https://hub.hku.hk/handle/10722/338688 https://doi.org/10.1016/j.jembe.2021.151683 |
| Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
| Αριθμός Καταχώρησης: | edsbas.48ED9372 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1016/j.jembe.2021.151683 |
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