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PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.

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Τίτλος: PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.
Συγγραφείς: Fink, Ryan, Imai, Shosei, Canziani, Annika, Kubitschke, Martin, Kistmacher, Vivien, Kretschmer, Anny, Sebastian, Eva, Ottens, Jana, Schmitz, Dietmar, Terai, Takuya, Gründemann, Jan, Hassan, Sami I, Gockel, Nala Eileen, Patriarchi, Tommaso, Reiner, Andreas, Fuhrmann, Martin, Campbell, Robert E, Masseck, Olivia Andrea, Lauer, German, Renken, Kim, Wietek, Jonas, Lamothe-Molina, Paul J, Fuhrmann, Falko, Mittag, Manuel, Ziebarth, Tim
Πηγή: Nature methods 23(5), 998 - 1010 (2026). doi:10.1038/s41592-026-03065-2
Στοιχεία εκδότη: Nature Publishing Group
Έτος έκδοσης: 2026
Θεματικοί όροι: info:eu-repo/classification/ddc/610, Animals, Calcium: metabolism, Calcium: analysis, Mice, Luminescent Proteins: chemistry, Luminescent Proteins: genetics, Luminescent Proteins: metabolism, Optogenetics: methods, Neurons: metabolism, Red Fluorescent Protein, Humans, Signal-To-Noise Ratio, HEK293 Cells, Calcium, Luminescent Proteins
Θέμα γεωγραφικό: DE
Περιγραφή: Genetically encoded calcium (Ca2+) indicators (GECIs) are essential tools for monitoring neuronal activity, but the performance of red fluorescent GECIs has remained limited. In particular, many red indicators are relatively dim, produce low signal-to-noise ratios and can undergo unwanted photoswitching when exposed to blue light, restricting their use in all-optical experiments that combine imaging with optogenetics or multicolor imaging. Here we show the development of PinkyCaMP, a Ca2+ sensor based on the bright red fluorescent protein mScarlet. PinkyCaMP exhibits markedly improved brightness, photostability and signal-to-noise ratio compared to existing red GECIs, while remaining fully compatible with blue-light-based optogenetic and dual-color imaging approaches. PinkyCaMP is well-tolerated by neurons, showing no detectable toxicity or aggregation, both in vitro and in vivo. PinkyCaMP enables a broad spectrum of imaging modalities, including single-photon methods, such as fiber photometry, widefield imaging and miniature microscopy imaging, as well as two-photon imaging in awake mice.
Τύπος εγγράφου: article in journal/newspaper
Γλώσσα: English
ISSN: 1548-7091
1548-7105
Relation: info:eu-repo/semantics/altIdentifier/issn/1548-7091; info:eu-repo/semantics/altIdentifier/issn/1548-7105; info:eu-repo/semantics/altIdentifier/pmid/pmid:42032066; https://pub.dzne.de/record/287261
Διαθεσιμότητα: https://pub.dzne.de/record/287261
https://pub.dzne.de/search?p=id:%22DZNE-2026-00522%22
Rights: info:eu-repo/semantics/openAccess
Αριθμός Καταχώρησης: edsbas.32A4BDA
Βάση Δεδομένων: BASE
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PubType: Academic Journal
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  Data: PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.
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  Data: Nature methods 23(5), 998 - 1010 (2026). doi:10.1038/s41592-026-03065-2
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  Data: Genetically encoded calcium (Ca2+) indicators (GECIs) are essential tools for monitoring neuronal activity, but the performance of red fluorescent GECIs has remained limited. In particular, many red indicators are relatively dim, produce low signal-to-noise ratios and can undergo unwanted photoswitching when exposed to blue light, restricting their use in all-optical experiments that combine imaging with optogenetics or multicolor imaging. Here we show the development of PinkyCaMP, a Ca2+ sensor based on the bright red fluorescent protein mScarlet. PinkyCaMP exhibits markedly improved brightness, photostability and signal-to-noise ratio compared to existing red GECIs, while remaining fully compatible with blue-light-based optogenetic and dual-color imaging approaches. PinkyCaMP is well-tolerated by neurons, showing no detectable toxicity or aggregation, both in vitro and in vivo. PinkyCaMP enables a broad spectrum of imaging modalities, including single-photon methods, such as fiber photometry, widefield imaging and miniature microscopy imaging, as well as two-photon imaging in awake mice.
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  Data: https://pub.dzne.de/record/287261<br />https://pub.dzne.de/search?p=id:%22DZNE-2026-00522%22
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