Academic Journal
PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.
| Τίτλος: | PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities. |
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| Συγγραφείς: | Fink, Ryan, Imai, Shosei, Canziani, Annika, Kubitschke, Martin, Kistmacher, Vivien, Kretschmer, Anny, Sebastian, Eva, Ottens, Jana, Schmitz, Dietmar, Terai, Takuya, Gründemann, Jan, Hassan, Sami I, Gockel, Nala Eileen, Patriarchi, Tommaso, Reiner, Andreas, Fuhrmann, Martin, Campbell, Robert E, Masseck, Olivia Andrea, Lauer, German, Renken, Kim, Wietek, Jonas, Lamothe-Molina, Paul J, Fuhrmann, Falko, Mittag, Manuel, Ziebarth, Tim |
| Πηγή: | Nature methods 23(5), 998 - 1010 (2026). doi:10.1038/s41592-026-03065-2 |
| Στοιχεία εκδότη: | Nature Publishing Group |
| Έτος έκδοσης: | 2026 |
| Θεματικοί όροι: | info:eu-repo/classification/ddc/610, Animals, Calcium: metabolism, Calcium: analysis, Mice, Luminescent Proteins: chemistry, Luminescent Proteins: genetics, Luminescent Proteins: metabolism, Optogenetics: methods, Neurons: metabolism, Red Fluorescent Protein, Humans, Signal-To-Noise Ratio, HEK293 Cells, Calcium, Luminescent Proteins |
| Θέμα γεωγραφικό: | DE |
| Περιγραφή: | Genetically encoded calcium (Ca2+) indicators (GECIs) are essential tools for monitoring neuronal activity, but the performance of red fluorescent GECIs has remained limited. In particular, many red indicators are relatively dim, produce low signal-to-noise ratios and can undergo unwanted photoswitching when exposed to blue light, restricting their use in all-optical experiments that combine imaging with optogenetics or multicolor imaging. Here we show the development of PinkyCaMP, a Ca2+ sensor based on the bright red fluorescent protein mScarlet. PinkyCaMP exhibits markedly improved brightness, photostability and signal-to-noise ratio compared to existing red GECIs, while remaining fully compatible with blue-light-based optogenetic and dual-color imaging approaches. PinkyCaMP is well-tolerated by neurons, showing no detectable toxicity or aggregation, both in vitro and in vivo. PinkyCaMP enables a broad spectrum of imaging modalities, including single-photon methods, such as fiber photometry, widefield imaging and miniature microscopy imaging, as well as two-photon imaging in awake mice. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| ISSN: | 1548-7091 1548-7105 |
| Relation: | info:eu-repo/semantics/altIdentifier/issn/1548-7091; info:eu-repo/semantics/altIdentifier/issn/1548-7105; info:eu-repo/semantics/altIdentifier/pmid/pmid:42032066; https://pub.dzne.de/record/287261 |
| Διαθεσιμότητα: | https://pub.dzne.de/record/287261 https://pub.dzne.de/search?p=id:%22DZNE-2026-00522%22 |
| Rights: | info:eu-repo/semantics/openAccess |
| Αριθμός Καταχώρησης: | edsbas.32A4BDA |
| Βάση Δεδομένων: | BASE |
| ISSN: | 15487091 15487105 |
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