Academic Journal
Mast cells are critical mediators of vaccine-induced Helicobacter clearance in the mouse model
| Title: | Mast cells are critical mediators of vaccine-induced Helicobacter clearance in the mouse model |
|---|---|
| Authors: | Velin, D., Bachmann, D., Bouzourene, H., Michetti, P. |
| Publication Year: | 2005 |
| Collection: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Subject Terms: | Administration, Intranasal Animals Bacterial Vaccines/*pharmacology Bone Marrow Cells/immunology/microbiology CD4-Positive T-Lymphocytes/immunology/microbiology Chymases Disease Models, Animal Female Gastric Mucosa/immunology/microbiology Helicobacter Infections/*immunology/*prevention & control Helicobacter felis/immunology Helicobacter pylori/*immunology Immunoglobulin E/immunology Male Mast Cells/*immunology/microbiology Mice Mice, Inbred C57BL Mice, Mutant Strains Neutrophils/immunology Proto-Oncogene Proteins c-kit/genetics RNA, Messenger/analysis Serine Endopeptidases/blood/genetics Urease/immunology |
| Description: | BACKGROUND & AIMS: Despite the proven ability of immunization to prevent Helicobacter infection in mouse models, the precise mechanism of protection has remained elusive. METHODS: We explored the cellular events associated with Helicobacter clearance from the stomach following vaccination by flow cytometry analysis and histological and molecular studies. RESULTS: Kinetic studies showed that the infection is undetectable in vaccinated mice at day 5 postbacterial challenge. Flow cytometry analysis showed that the percentages of mast cells (CD3 - CD117 + ) increased in the lymphoid cells isolated from the stomach at day 4 postchallenge in urease + cholera toxin (CT)-vaccinated mice in comparison with mice administered with CT alone (9.4% +/- 4.4% and 3.1% +/- 1%, respectively, for vaccinated and CT administered, n = 5; P < .01). Quantitative PCR analysis showed an increased messenger RNA (mRNA) expression of the mast cell proteases 1 and 2 at day 5 postchallenge in the stomach of vaccinated mice. In contrast to wild-type mice, mast cell-deficient mice (W/W v mice) were not protected from H felis colonization after vaccination. Indeed only 1 out of 12 vaccinated W/W v mice showed a negative urease test. Remarkably, vaccinated W/W v mice reconstituted with cultured bone marrow-derived mast cells recovered the ability to clear the infection after vaccination (8 out of 10 mast cell-reconstituted mice showed negative urease tests [ P < .006 as compared with wild-type mice]). CONCLUSIONS: These experiments show that mast cells are, unexpectedly, critical mediators of anti- Helicobacter vaccination. |
| Document Type: | article in journal/newspaper |
| Language: | unknown |
| ISSN: | 0016-5085 |
| Relation: | Gastroenterology; https://iris.unil.ch/handle/iris/69687; serval:BIB_0C18B5EDBB1A; 000230423100019 |
| DOI: | 10.1053/j.gastro.2005.04.010 |
| Availability: | https://iris.unil.ch/handle/iris/69687 https://doi.org/10.1053/j.gastro.2005.04.010 |
| Accession Number: | edsbas.276DC17 |
| Database: | BASE |
| ISSN: | 00165085 |
|---|---|
| DOI: | 10.1053/j.gastro.2005.04.010 |