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Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing

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Title: Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing
Contributors: Y. Park, Y. S. Kim, S. I. Kim, H. Kim, H. S. Kim, Kim, Soon Il, Kim, Yu Seun, Kim, Hyon Suk
Publication Year: 2012
Subject Terms: Alleles, Behcet Syndrome/diagnosis, Behcet Syndrome/genetics, Behcet Syndrome/immunology, Genotype, HLA-B51 Antigen/genetics, HLA-B51 Antigen/immunology, Histocompatibility Testing/methods, Humans, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, Behcet’s disease, human leukocyteantigen-B51, real-time polymerase chainreaction, sequence-based typing, sequence-specific priming polymerasechain reactio
Description: The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories. ; open
Document Type: article in journal/newspaper
Language: unknown
Relation: TISSUE ANTIGENS; J02731; https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931; T201203589; 30013
DOI: 10.1111/j.1399-0039.2012.01942.x/abstract
Availability: https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931
http://onlinelibrary.wiley.com/doi/10.1111/j.1399-0039.2012.01942.x/abstract
Rights: CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/
Accession Number: edsbas.23DE45B5
Database: BASE
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  – Url: https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931#
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PubType: Academic Journal
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing
– Name: Author
  Label: Contributors
  Group: Au
  Data: Y. Park<br />Y. S. Kim<br />S. I. Kim<br />H. Kim<br />H. S. Kim<br />Kim, Soon Il<br />Kim, Yu Seun<br />Kim, Hyon Suk
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2012
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Alleles%22">Alleles</searchLink><br /><searchLink fieldCode="DE" term="%22Behcet+Syndrome%2Fdiagnosis%22">Behcet Syndrome/diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Behcet+Syndrome%2Fgenetics%22">Behcet Syndrome/genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Behcet+Syndrome%2Fimmunology%22">Behcet Syndrome/immunology</searchLink><br /><searchLink fieldCode="DE" term="%22Genotype%22">Genotype</searchLink><br /><searchLink fieldCode="DE" term="%22HLA-B51+Antigen%2Fgenetics%22">HLA-B51 Antigen/genetics</searchLink><br /><searchLink fieldCode="DE" term="%22HLA-B51+Antigen%2Fimmunology%22">HLA-B51 Antigen/immunology</searchLink><br /><searchLink fieldCode="DE" term="%22Histocompatibility+Testing%2Fmethods%22">Histocompatibility Testing/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22Real-Time+Polymerase+Chain+Reaction%22">Real-Time Polymerase Chain Reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Sensitivity+and+Specificity%22">Sensitivity and Specificity</searchLink><br /><searchLink fieldCode="DE" term="%22Sequence+Analysis%22">Sequence Analysis</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Behcet%27s+disease%22">Behcet’s disease</searchLink><br /><searchLink fieldCode="DE" term="%22human+leukocyteantigen-B51%22">human leukocyteantigen-B51</searchLink><br /><searchLink fieldCode="DE" term="%22real-time+polymerase+chainreaction%22">real-time polymerase chainreaction</searchLink><br /><searchLink fieldCode="DE" term="%22sequence-based+typing%22">sequence-based typing</searchLink><br /><searchLink fieldCode="DE" term="%22sequence-specific+priming+polymerasechain+reactio%22">sequence-specific priming polymerasechain reactio</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories. ; open
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article in journal/newspaper
– Name: Language
  Label: Language
  Group: Lang
  Data: unknown
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: TISSUE ANTIGENS; J02731; https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931; T201203589; 30013
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1111/j.1399-0039.2012.01942.x/abstract
– Name: URL
  Label: Availability
  Group: URL
  Data: https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931<br />http://onlinelibrary.wiley.com/doi/10.1111/j.1399-0039.2012.01942.x/abstract
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  Data: CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/
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  Label: Accession Number
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  Data: edsbas.23DE45B5
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RecordInfo BibRecord:
  BibEntity:
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      – Type: doi
        Value: 10.1111/j.1399-0039.2012.01942.x/abstract
    Languages:
      – Text: unknown
    Subjects:
      – SubjectFull: Alleles
        Type: general
      – SubjectFull: Behcet Syndrome/diagnosis
        Type: general
      – SubjectFull: Behcet Syndrome/genetics
        Type: general
      – SubjectFull: Behcet Syndrome/immunology
        Type: general
      – SubjectFull: Genotype
        Type: general
      – SubjectFull: HLA-B51 Antigen/genetics
        Type: general
      – SubjectFull: HLA-B51 Antigen/immunology
        Type: general
      – SubjectFull: Histocompatibility Testing/methods
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Real-Time Polymerase Chain Reaction
        Type: general
      – SubjectFull: Sensitivity and Specificity
        Type: general
      – SubjectFull: Sequence Analysis
        Type: general
      – SubjectFull: DNA
        Type: general
      – SubjectFull: Behcet’s disease
        Type: general
      – SubjectFull: human leukocyteantigen-B51
        Type: general
      – SubjectFull: real-time polymerase chainreaction
        Type: general
      – SubjectFull: sequence-based typing
        Type: general
      – SubjectFull: sequence-specific priming polymerasechain reactio
        Type: general
    Titles:
      – TitleFull: Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing
        Type: main
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            NameFull: Y. Park
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            NameFull: Y. S. Kim
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            NameFull: S. I. Kim
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            NameFull: H. Kim
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            NameFull: Kim, Soon Il
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            NameFull: Kim, Yu Seun
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            NameFull: Kim, Hyon Suk
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              Y: 2012
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