Academic Journal
Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing
| Τίτλος: | Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing |
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| Συνεισφορές: | Y. Park, Y. S. Kim, S. I. Kim, H. Kim, H. S. Kim, Kim, Soon Il, Kim, Yu Seun, Kim, Hyon Suk |
| Έτος έκδοσης: | 2012 |
| Θεματικοί όροι: | Alleles, Behcet Syndrome/diagnosis, Behcet Syndrome/genetics, Behcet Syndrome/immunology, Genotype, HLA-B51 Antigen/genetics, HLA-B51 Antigen/immunology, Histocompatibility Testing/methods, Humans, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, Behcet’s disease, human leukocyteantigen-B51, real-time polymerase chainreaction, sequence-based typing, sequence-specific priming polymerasechain reactio |
| Περιγραφή: | The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories. ; open |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | unknown |
| Relation: | TISSUE ANTIGENS; J02731; https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931; T201203589; 30013 |
| DOI: | 10.1111/j.1399-0039.2012.01942.x/abstract |
| Διαθεσιμότητα: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91931 http://onlinelibrary.wiley.com/doi/10.1111/j.1399-0039.2012.01942.x/abstract |
| Rights: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ |
| Αριθμός Καταχώρησης: | edsbas.23DE45B5 |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.1111/j.1399-0039.2012.01942.x/abstract |
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