Academic Journal
Determination of Arsenobetaine in Fish Tissue by Species Specific Isotope Dilution LC-LTQ-Orbitrap-MS and Standard Addition LC-ICPMS
| Τίτλος: | Determination of Arsenobetaine in Fish Tissue by Species Specific Isotope Dilution LC-LTQ-Orbitrap-MS and Standard Addition LC-ICPMS |
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| Συγγραφείς: | Anthony Windust, Lu Yang, Sezgin Bakırdere, Laurent Ouerdane, Margaret McCooeye, Scott Willie, Paulette Maxwell, Zoltán Mester, Jianfu Ding |
| Στοιχεία εκδότη: | American Chemical Society (ACS) |
| Έτος έκδοσης: | 2025 |
| Θεματικοί όροι: | exact matching, Indicator Dilution Techniques, inductively coupled plasma, mass bias correction, 01 natural sciences, Arsenicals, Mass Spectrometry, arsenobetaine, measured results, scan mode, Isotopes, indicator dilution curve, species-specific isotope dilution, reference standards, inductively coupled plasma mass spectrometry, detection limits, fish tissue, mass spectrometers, Fishes, standard, methodology, Orbitrap, organoarsenic derivative, resonance, Calibration, standards, pollutant, Environmental Pollutants, deionized water, method validations |
| Περιγραφή: | An accurate and precise method for the determination of arsenobetaine (AsB, (CH(3))(3)(+)AsCH(2)COO(-)) in fish samples using exact matching species specific isotope dilution (ID) liquid chromatography LTQ-Orbitrap mass spectrometry (LC-LTQ-Orbitrap-MS) and standard addition LC inductively coupled plasma mass spectrometry (LC-ICPMS) is described. Samples were extracted by sonication for 30 min with high purity deionized water. An in-house synthesized (13)C enriched AsB spike was used for species specific ID analysis whereas natural abundance AsB, synthesized and characterized by quantitative (1)H NMR (nuclear magnetic resonance spectroscopy), was used for reverse ID and standard addition LC-ICPMS. With the LTQ-Orbitrap-MS instrument in scan mode (m/z 170-190) and resolution set at 7500, the intensities of [M + H](+) ions at m/z of 179.0053 and 180.0087 were used to calculate the 179.0053/180.0087 ion ratio for quantification of AsB in fish tissues. To circumvent potential difficulty in mass bias correction, an exact matching approach was applied. A quantitatively prepared mixture of the natural abundance AsB standard and the enriched spike to give a ratio near one was used for mass bias correction. Concentrations of 9.65 ± 0.24 and 11.39 ± 0.39 mg kg(-1) (expanded uncertainty, k = 2) for AsB in two fish samples of fish1 and fish2, respectively, were obtained by ID LC-LTQ-Orbitrap-MS. These results are in good agreement with those obtained by standard addition LC-ICPMS, 9.56 ± 0.32 and 11.26 ± 0.44 mg kg(-1) (expanded uncertainty, k = 2), respectively. Fish CRM DORM-2 was used for method validation and measured results of 37.9 ± 1.8 and 38.7 ± 0.66 mg kg(-1) (expanded uncertainty, k = 2) for AsB obtained by standard addition LC-ICPMS and ID LC-LTQ-Orbitrap-MS, respectively, are in good agreement with the certified value of 39.0 ± 2.6 mg kg(-1) (expanded uncertainty, k = 2). Detection limits of 0.011 and 0.033 mg kg(-1) for AsB with LC-ICPMS and ID LC-LTQ-Orbitrap-MS, respectively, were obtained demonstrating that ... |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | unknown |
| ISSN: | 21452903 |
| Relation: | Analytical Chemistry; https://hdl.handle.net/20.500.12628/21696; 000289956800024; 3371; 3378; 83 |
| DOI: | 10.1021/ac103258m |
| Διαθεσιμότητα: | https://hdl.handle.net/20.500.12628/21696 https://doi.org/10.1021/ac103258m |
| Rights: | CLOSED |
| Αριθμός Καταχώρησης: | edsbas.2323B224 |
| Βάση Δεδομένων: | BASE |
| ISSN: | 21452903 |
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| DOI: | 10.1021/ac103258m |