Dissertation/Thesis
VOLUMETRIC IMAGING OF CELLULAR DYNAMICS USING LIGHT-SHEET MICROSCOPY: FROM SUBCELLULAR STRUCTURES TO WHOLE ORGANISMS
| Τίτλος: | VOLUMETRIC IMAGING OF CELLULAR DYNAMICS USING LIGHT-SHEET MICROSCOPY: FROM SUBCELLULAR STRUCTURES TO WHOLE ORGANISMS |
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| Συγγραφείς: | Prince, Md Nasful Huda |
| Πηγή: | Optical Science and Engineering ETDs |
| Στοιχεία εκδότη: | UNM Digital Repository |
| Έτος έκδοσης: | 2025 |
| Συλλογή: | UNM Digital Repository (The University of New Mexico) |
| Θεματικοί όροι: | Light-sheet Microscopy, Volumetric Imaging, Remote Focusing, Oblique Plane, Isotropic Imaging, Atomic, Molecular and Optical Physics, Bioimaging and Biomedical Optics, Biomedical Devices and Instrumentation, Biotechnology, Optics, Other Engineering |
| Περιγραφή: | Advancing biomedical imaging requires platforms that combine high-resolution, speed, and stability across biological scales. This dissertation presents three innovations designed to address limitations of conventional light-sheet and oblique plane imaging. First, we introduce Reflected Inline Detection in Epi-Oblique Plane Microscopy (RIDE-OPM), a compact and drift-resistant system that eliminates a tertiary detection path while maintaining alignment flexibility, enabling robust long-term live imaging. Second, we present a high-speed Axially Swept Light-Sheet Microscopy (ASLM) platform for cleared tissues, using dual foci and synchronized sweeping to achieve isotropic sub-micron resolution at up to 40 frames-per-second, a fourfold improvement over standard ASLM. Integrated with a deep-learning tissue boundary detector, it enables efficient 3D imaging. Finally, we propose a remote focusing architecture that ensures consistent magnification and 4f geometry across objectives, enabling seamless multi-scale imaging. Together, these methods establish a flexible framework for next-generation microscopy with broad applications in live, cleared, and multi-resolution imaging. |
| Τύπος εγγράφου: | Thesis |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| Relation: | https://digitalrepository.unm.edu/ose_etds/114; https://digitalrepository.unm.edu/context/ose_etds/article/1116/viewcontent/Dissertation_Prince_r4.pdf |
| Διαθεσιμότητα: | https://digitalrepository.unm.edu/ose_etds/114 https://digitalrepository.unm.edu/context/ose_etds/article/1116/viewcontent/Dissertation_Prince_r4.pdf |
| Αριθμός Καταχώρησης: | edsbas.21DF84E8 |
| Βάση Δεδομένων: | BASE |
| Η περιγραφή δεν είναι διαθέσιμη |