Conference
Presentation_1_Whole Genome Analysis Detects the Emergence of a Single Salmonella enterica Serovar Chester Clone in Japan’s Kanto Region.PPTX
| Title: | Presentation_1_Whole Genome Analysis Detects the Emergence of a Single Salmonella enterica Serovar Chester Clone in Japan’s Kanto Region.PPTX |
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| Authors: | Naoshi Ando, Tsuyoshi Sekizuka, Eiji Yokoyama, Yoshiyuki Aihara, Noriko Konishi, Yuko Matsumoto, Kumiko Ishida, Koo Nagasawa, Nathalie Jourdan-Da Silva, Motoi Suzuki, Hirokazu Kimura, Simon Le Hello, Koichi Murakami, Makoto Kuroda, Shinichiro Hirai, Setsuko Fukaya |
| Publication Year: | 2021 |
| Collection: | Frontiers: Figshare |
| Subject Terms: | Microbiology, Microbial Genetics, Microbial Ecology, Mycology, Salmonella Chester, emerging, molecular epidemiological analysis, population genetic analysis, whole genome sequence data, single-nucleotide polymorphisms, selection pressure |
| Description: | In Japan’s Kanto region, the number of Salmonella enterica serovar Chester infections increased temporarily between 2014 and 2016. Concurrently with this temporal increase in the Kanto region, S. Chester isolates belonging to one clonal group were causing repetitive outbreaks in Europe. A recent study reported that the European outbreaks were associated with travelers who had been exposed to contaminated food in Morocco, possibly seafood. Because Japan imports a large amount of seafood from Morocco, we aimed to establish whether the temporal increase in S. Chester infections in the Kanto region was associated with imported Moroccan seafood. Short sequence reads from the whole-genome sequencing of 47 S. Chester isolates from people in the Kanto region (2014–2016), and the additional genome sequences from 58 isolates from the European outbreaks, were analyzed. The reads were compared with the complete genome sequence from a S. Chester reference strain, and 347 single nucleotide polymorphisms (SNPs) were identified. These SNPs were used in this study. Cluster and Bayesian cluster analyses showed that the Japanese and European isolates fell into two different clusters. Therefore, Φ PT and IAS values were calculated to evaluate genetic differences between these clusters. The results revealed that the Japanese and European isolates were genetically distinct populations. Our root-to-tip analysis showed that the Japanese isolates originating from one clone had accumulated mutations, suggesting that an emergence of this organism occurred. A minimum spanning tree analysis demonstrated no correlation between genetic and geographical distances in the Japanese isolates, suggesting that the emergence of the serovar in the Kanto region did not involve person-to-person contact; rather, it occurred through food consumption. The d N /d S ratio indicated that the Japanese strain has evolved under positive selection pressure. Generally, a population of bacterial clones in a reservoir faces negative selection pressure. Therefore, ... |
| Document Type: | conference object |
| Language: | unknown |
| Relation: | https://figshare.com/articles/presentation/Presentation_1_Whole_Genome_Analysis_Detects_the_Emergence_of_a_Single_Salmonella_enterica_Serovar_Chester_Clone_in_Japan_s_Kanto_Region_PPTX/15059220 |
| DOI: | 10.3389/fmicb.2021.705679.s001 |
| Availability: | https://doi.org/10.3389/fmicb.2021.705679.s001 https://figshare.com/articles/presentation/Presentation_1_Whole_Genome_Analysis_Detects_the_Emergence_of_a_Single_Salmonella_enterica_Serovar_Chester_Clone_in_Japan_s_Kanto_Region_PPTX/15059220 |
| Rights: | CC BY 4.0 |
| Accession Number: | edsbas.20D84425 |
| Database: | BASE |
| DOI: | 10.3389/fmicb.2021.705679.s001 |
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