Academic Journal
Oil-Adjuvanted Polyvalent Formalin-Killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami
| Τίτλος: | Oil-Adjuvanted Polyvalent Formalin-Killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami |
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| Συγγραφείς: | Rozi, Tyasningsih, Wiwiek, Rahmahani, Jola, Aksono, Eduardus Bimo, Yunus, Muchammad, Arif, Mohammad Anam Al, Kuncorojakti, Suryo, Nindarwi, Daruti Dinda, Sari, Putri Desi Wulan, Dewi, Nina Nurmalia, Satyantini, Woro Hastuti, Santanumurti, Muhammad Browijoyo, Wisudyawati, Dita, Azmai, Mohammad Noor Amal, Salleh, Annas, Salim, Gazali, Suwarno |
| Στοιχεία εκδότη: | Faculty of Fisheries and Marine Universitas Airlangga |
| Έτος έκδοσης: | 2026 |
| Συλλογή: | Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository |
| Θεματικοί όροι: | Food Science, Ecology, Evolution, Behavior and Systematics, Aquatic Science |
| Περιγραφή: | Motile Aeromonas septicaemia (MAS), predominantly associated with Aeromonas hydrophila, remains a major constraint in giant gourami (Osphronemus goramy) aquaculture. This study evaluated formalin-inactivated A. hydrophila vaccines prepared from MAS-associated field isolates, comparing a monovalent formulation (P2), a non-adjuvanted polyvalent formulation (P3), and an oil-adjuvanted polyvalent formulation (P4) against PBS controls (P1). A total of 240 fish were used (60 per treatment) and assigned to two parallel cohorts (immunology and survival/challenge). Immune endpoints (agglutinating titres, NBT activity, and splenic il-1β and ifn-γ transcription) were assessed on days 7, 14, 21, 35, and 42 post-vaccination. The survival cohort was challenged intraperitoneally at day 21 with a homologous A. hydrophila strain and monitored for 14 days post-challenge. Vaccination was clinically well tolerated and improved survival relative to controls, with P4 showing the highest protection (RPS 81.8%). Agglutinating titres differed by treatment and time; at the peak sampling point (day 35), mean titres in P4 were ~200-fold higher than in P1, and model contrasts indicated significant differences versus controls (p<0.001). Splenic il-1β and ifn-γ transcript levels were higher in vaccinated groups than in controls at later time points. These findings support further evaluation of an oil-adjuvanted polyvalent inactivated A. hydrophila vaccine for gourami, including dose optimisation, extended safety assessment, heterologous challenge, and field validation. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | text |
| Γλώσσα: | English |
| ISSN: | 2085-5842 |
| Relation: | http://psasir.upm.edu.my/id/eprint/124926/1/124926.pdf; Rozi and Tyasningsih, Wiwiek and Rahmahani, Jola and Aksono, Eduardus Bimo and Yunus, Muchammad and Arif, Mohammad Anam Al and Kuncorojakti, Suryo and Nindarwi, Daruti Dinda and Sari, Putri Desi Wulan and Dewi, Nina Nurmalia and Satyantini, Woro Hastuti and Santanumurti, Muhammad Browijoyo and Wisudyawati, Dita and Azmai, Mohammad Noor Amal and Salleh, Annas and Salim, Gazali and Suwarno (2026) Oil-Adjuvanted Polyvalent Formalin-Killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami. Jurnal Ilmiah Perikanan dan Kelautan, 18 (1). pp. 17-40. ISSN 2085-5842; eISSN: 2528-0759 |
| Διαθεσιμότητα: | http://psasir.upm.edu.my/id/eprint/124926/ http://psasir.upm.edu.my/id/eprint/124926/1/124926.pdf https://e-journal.unair.ac.id/JIPK/article/view/82866 |
| Rights: | cc_by_nc_sa_4 |
| Αριθμός Καταχώρησης: | edsbas.1EDDA823 |
| Βάση Δεδομένων: | BASE |
| ISSN: | 20855842 |
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