Academic Journal
The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions
| Title: | The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions |
|---|---|
| Authors: | Pichon, Xavier, Moissoglu, Konstadinos, Coleno, Emeline, Wang, Tianhong, Imbert, Arthur, Robert, Marie-Cecile, Peter, Marion, Chouaib, Racha, Walter, Thomas, Mueller, Florian, Zibara, Kazem, Bertrand, Edouard, Mili, Stavroula |
| Contributors: | Institut de Génétique Moléculaire de Montpellier (IGMM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Equipe labellisée Ligue contre le Cancer, National Cancer Institute Bethesda (NCI-NIH), National Institutes of Health Bethesda, MD, USA (NIH), Institut de génétique humaine (IGH), Cancer et génome: Bioinformatique, biostatistiques et épidémiologie d'un système complexe, Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut Curie Paris -Institut National de la Santé et de la Recherche Médicale (INSERM), الجامعة اللبنانية بيروت = Lebanese University Beirut = Université libanaise Beyrouth (LU / ULB), Imagerie et Modélisation - Imaging and Modeling, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI), Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), This project was supported by France BioImaging (ANR-10-INBS-04), the Agence Nationale de la Recherche (ANR-11-BSV8-018-02, ANR-14-CE10-0018-01 and ANR-19-CE12-0007-03), and the Ligue Nationale Contre le Cancer and the Fondation pour la Recherche Médicale. This work was supported by the Labex EpiGenMed, from the framework "Investissements d’avenir". This work was supported in part by the Intramural Research Program of the National Cancer Institute, NIH., ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-11-BSV8-0018,EJCbirth,Impacts de la transcription par l'ARN polymérase II sur l'assemblage de l'EJC(2011), ANR-14-CE10-0018,HI-FISH,Etude systématique de l'expression des gènes à l'échelle des molécules uniques d'ARN(2014), ANR-19-CE12-0007,TRANSFACT,Caracterisation des foyers de traduction: de nouvelles structures qui organisement finement le cytoplasme(2019), ANR-10-LABX-0012,EpiGenMed,From Genome and Epigenome to Molecular Medicine: turning new paradigms in biology into the therapeutic strategies of tomorrow(2010) |
| Source: | ISSN: 1355-8382. |
| Publisher Information: | CCSD Cold Spring Harbor Laboratory Press |
| Publication Year: | 2021 |
| Collection: | Inserm: HAL (Institut national de la santé et de la recherche médicale) |
| Subject Terms: | RNA transport, cytoplasmic protrusions, local translation, RNA localization, [SDV]Life Sciences [q-bio] |
| Description: | International audience ; RNA localization and local translation are important for numerous cellular functions. In mammals, a class of mRNAs localize to cytoplasmic protrusions in an APC-dependent manner, with roles during cell migration. Here, we investigated this localization mechanism. We found that the KIF1C motor interacts with APC-dependent mRNAs and is required for their localization. Live cell imaging revealed rapid, active transport of single mRNAs over long distances that requires both microtubules and KIF1C. Two color imaging directly revealed single mRNAs transported by single KIF1C motors, with the 3’UTR being sufficient to trigger KIF1C-dependent RNA transport and localization. Moreover, KIF1C remained associated with peripheral, multimeric RNA clusters and was required for their formation. These results reveal a widespread RNA transport pathway in mammalian cells, in which the KIF1C motor has a dual role in transporting RNAs and clustering them within cytoplasmic protrusions. Interestingly, KIF1C also transports its own mRNA suggesting a possible feedback loop acting at the level of mRNA transport. |
| Document Type: | article in journal/newspaper |
| Language: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34493599; PUBMED: 34493599 |
| DOI: | 10.1261/rna.078576.120 |
| Availability: | https://hal.science/hal-03371977 https://hal.science/hal-03371977v1/document https://hal.science/hal-03371977v1/file/2021-Pichon_RNA.pdf https://doi.org/10.1261/rna.078576.120 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: | edsbas.137CA26 |
| Database: | BASE |
| DOI: | 10.1261/rna.078576.120 |
|---|