Academic Journal
Interferon-gamma producing CD4+ T cells quantified by flow cytometry as early markers for Mycobacterium avium ssp. paratuberculosis infection in cattle
| Title: | Interferon-gamma producing CD4+ T cells quantified by flow cytometry as early markers for Mycobacterium avium ssp. paratuberculosis infection in cattle |
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| Authors: | Hakan Bulun, Philip S. Bridger, Simone Schillinger, Ömer Akineden, Stefanie A. Barth, Marta Fischer, Manfred Henrich, Torsten Seeger, Klaus Doll, Michael Bülte, Rolf Bauerfeind, Christian Menge |
| Source: | Vet Res Veterinary Research, Vol 55, Iss 1, Pp 1-21 (2024) |
| Publisher Information: | Springer Science and Business Media LLC, 2024. |
| Publication Year: | 2024 |
| Subject Terms: | Flow Cytometry/veterinary [MeSH], Mycobacterium avium subsp. paratuberculosis/immunology [MeSH], Cattle Diseases/diagnosis [MeSH], Mycobacterium avium subsp. paratuberculosis/physiology [MeSH], IFN-γ, Interferon-gamma/metabolism [MeSH], Cattle Diseases/microbiology [MeSH], Animals [MeSH], Flow Cytometry/methods [MeSH], Paratuberculosis/immunology [MeSH], T cell, CD4-Positive T-Lymphocytes/immunology [MeSH], Paratuberculosis/diagnosis [MeSH], Johne's disease, flow cytometry, Paratuberculosis/microbiology [MeSH], Cattle [MeSH], Biomarkers [MeSH], Cattle Diseases/immunology [MeSH], Research Article, CD4-Positive T-Lymphocytes, 0301 basic medicine, Veterinary medicine, Cattle Diseases, 04 agricultural and veterinary sciences, Flow Cytometry, 3. Good health, Mycobacterium avium subsp. paratuberculosis, 0403 veterinary science, Interferon-gamma, 03 medical and health sciences, SF600-1100, Paratuberculosis, Animals, Cattle, M. avium ssp. paratuberculosis, Biomarkers |
| Description: | Current diagnostic methods for Johne’s disease in cattle allow reliable detection of infections with Mycobacterium avium ssp. paratuberculosis (MAP) not before animals are 2 years of age. Applying a flow cytometry-based approach (FCA) to quantify a MAP-specific interferon-gamma (IFN-γ) response in T cell subsets, the present study sought to monitor the kinetics of the cell-mediated immune response in experimentally infected calves. Six MAP-negative calves and six calves, orally inoculated with MAP at 10 days of age, were sampled every 4 weeks for 52 weeks post-inoculation (wpi). Peripheral blood mononuclear cells (PBMC) were stimulated with either purified protein derivatives (PPD) or whole cell sonicates derived from MAP (WCSj), M. avium ssp. avium or M. phlei for 6 days followed by labeling of intracellular IFN-γ in CD4+ and CD8+ T cells. No antigen-specific IFN-γ production was detectable in CD8+ cells throughout and the responses of CD4+ cells of MAP-infected and control calves were similar up to 12 wpi. However, the mean fluorescence intensity (MFI) for the detection of IFN-γ in CD4+ cells after WCSj antigen stimulation allowed for a differentiation of animal groups from 16 wpi onwards. This approach had a superior sensitivity (87.8%) and specificity (86.8%) to detect infected animals from 16 wpi onwards, i.e., in an early infection stage, as compared to the IFN-γ release assay (IGRA). Quantification of specific IFN-γ production at the level of individual CD4+ cells may serve, therefore, as a valuable tool to identify MAP-infected juvenile cattle. |
| Document Type: | Article Other literature type |
| Language: | English |
| ISSN: | 1297-9716 |
| DOI: | 10.1186/s13567-024-01324-8 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/38822400 https://doaj.org/article/3b4918712cd642e0a10fcef8f8c34124 https://repository.publisso.de/resource/frl:6519781 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (http://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
| Accession Number: | edsair.doi.dedup.....e9c1956f87337f392a782bf78ef622a3 |
| Database: | OpenAIRE |
| ISSN: | 12979716 |
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| DOI: | 10.1186/s13567-024-01324-8 |