Academic Journal
Cut&tag: a powerful epigenetic tool for chromatin profiling
| Title: | Cut&tag: a powerful epigenetic tool for chromatin profiling |
|---|---|
| Authors: | Peipei Li, Zhongheng Fu, Sanjie Jiang, Yiwen Sun, Shichun Zheng, Liang Zong |
| Source: | Epigenetics Epigenetics, Vol 19, Iss 1 (2024) |
| Publisher Information: | Informa UK Limited, 2023. |
| Publication Year: | 2023 |
| Subject Terms: | single-cell CUT&Tag, Epigenomics, Bivalent chromatin, Epigenetic Modifications and Their Functional Implications, Review, Tn5 tagmentation, QH426-470, DNA Nanotechnology and Bioanalytical Applications, Gene, Epigenesis, Genetic, Computational biology, Chromatin remodeling, Epigenome, Biochemistry, Genetics and Molecular Biology, Genetics, CUT&Tag, Molecular Biology, Biology, Chromatin Structure, chromatin profiling, DNA methylation, epigenetics, High-Throughput Nucleotide Sequencing, Life Sciences, Promoter, ChIP-sequencing, DNA Methylation, Comprehensive Integration of Single-Cell Transcriptomic Data, Chromatin immunoprecipitation, Chromatin, Epigenetic Reprogramming, Histone, FOS: Biological sciences, Epigenetics, Gene expression |
| Description: | Analysis of transcription factors and chromatin modifications at the genome-wide level provides insights into gene regulatory processes, such as transcription, cell differentiation and cellular response. Chromatin immunoprecipitation is the most popular and powerful approach for mapping chromatin, and other enzyme-tethering techniques have recently become available for living cells. Among these, CUT&Tag is a relatively novel chromatin profiling method that has rapidly gained popularity in the field of epigenetics since 2019. It has also been widely adapted to map chromatin modifications and TFs in different species, illustrating the association of these chromatin epitopes with various physiological and pathological processes. Scalable single-cell CUT&Tag can be combined with distinct platforms to distinguish cellular identity, epigenetic features and even spatial chromatin profiling. In addition, CUT&Tag has been developed as a strategy for joint profiling of the epigenome, transcriptome or proteome on the same sample. In this review, we will mainly consolidate the applications of CUT&Tag and its derivatives on different platforms, give a detailed explanation of the pros and cons of this technique as well as the potential development trends and applications in the future. |
| Document Type: | Article Other literature type |
| Language: | English |
| ISSN: | 1559-2308 1559-2294 |
| DOI: | 10.1080/15592294.2023.2293411 |
| DOI: | 10.22541/au.169203623.39699591/v1 |
| DOI: | 10.60692/s1m77-d9651 |
| DOI: | 10.60692/sk5na-9hp55 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/38105608 https://doaj.org/article/02b2c60adaab4d7bac17659c1069b253 |
| Rights: | CC BY URL: http://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
| Accession Number: | edsair.doi.dedup.....dec5f72b80dbc4fc61e1a2f2a73a3a97 |
| Database: | OpenAIRE |
| ISSN: | 15592308 15592294 |
|---|---|
| DOI: | 10.1080/15592294.2023.2293411 |